A combinatorial approach of comprehensive QTL-based comparative genome mapping and transcript profiling identified a seed weight-regulating candidate gene in chickpea
OAR@ICRISAT
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Relation |
http://oar.icrisat.org/8892/
http://dx.doi.org/10.1038/srep09264 10.1038/srep09264 |
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Title |
A combinatorial approach of comprehensive QTL-based comparative genome mapping and transcript profiling identified a seed weight-regulating candidate gene in chickpea
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Creator |
Bajaj, D
Upadhyaya, H D Khan, Y Das, S Badoni, S Shree, T Kumar, V Tripathi, S Gowda, C L L Singh, S Sharma, S Tyagi, A K Chattopdhyay, D Parida, S K |
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Subject |
Chickpea
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Description |
High experimental validation/genotyping success rate (94–96%) and intra-specific polymorphic potential (82–96%) of 1536 SNP and 472 SSR markers showing in silico polymorphism between desi ICC 4958 and kabuli ICC 12968 chickpea was obtained in a 190 mapping population (ICC 4958 × ICC 12968) and 92 diverse desi and kabuli genotypes. A high-density 2001 marker-based intra-specific genetic linkage map comprising of eight LGs constructed is comparatively much saturated (mean map-density: 0.94 cM) in contrast to existing intra-specific genetic maps in chickpea. Fifteen robust QTLs (PVE: 8.8–25.8% with LOD: 7.0–13.8) associated with pod and seed number/plant (PN and SN) and 100 seed weight (SW) were identified and mapped on 10 major genomic regions of eight LGs. One of 126.8 kb major genomic region harbouring a strong SW-associated robust QTL (Caq'SW1.1: 169.1–171.3 cM) has been delineated by integrating high-resolution QTL mapping with comprehensive marker-based comparative genome mapping and differential expression profiling. This identified one potential regulatory SNP (G/A) in the cis-acting element of candidate ERF (ethylene responsive factor) TF (transcription factor) gene governing seed weight in chickpea. The functionally relevant molecular tags identified have potential to be utilized for marker-assisted genetic improvement of chickpea.
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Publisher |
Nature Publishing Group
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Date |
2015-03
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Rights |
—
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Identifier |
http://oar.icrisat.org/8892/1/srep09264.pdf
Bajaj, D and Upadhyaya, H D and Khan, Y and Das, S and Badoni, S and Shree, T and Kumar, V and Tripathi, S and Gowda, C L L and Singh, S and Sharma, S and Tyagi, A K and Chattopdhyay, D and Parida, S K (2015) A combinatorial approach of comprehensive QTL-based comparative genome mapping and transcript profiling identified a seed weight-regulating candidate gene in chickpea. Scientific Reports, 5 (9264). pp. 1-14. ISSN 2045-2322 |
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