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Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes

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Relation http://oar.icrisat.org/8986/
http://dx.doi.org/10.1016/j.plantsci.2015.08.013
10.1016/j.plantsci.2015.08.013
 
Title Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes
 
Creator Janila, P
Pandey, M K
Shasidhar, Y
Variath, M T
Sriswathi, M
Khera, P
Manohar, S S
Nagesh, P
Vishwakarma, M K
Mishra, G P
Radhakrishnan, T
Manivannan, N
Dobariya, K L
Vasanthi, R P
Varshney, R K
 
Subject Groundnut
 
Description High oleate peanuts have two marketable benefits, health benefits to consumers and extended shelf life of peanut products. Two mutant alleles present on linkage group a09 ( ahFAD2A) and b09 ( ahFAD2B) control composition of three major fatty acids, oleic, linoleic and palmitic acids which together determine peanut oil quality. In conventional breeding, selection for fatty acid composition is delayed to advanced generations. However by using DNA markers, breeders can reject large number of plants in early generations and therefore can optimize time and resources. Here, two approaches of molecular breeding namely marker-assisted backcrossing (MABC) and marker-assisted selection (MAS) were employed to transfer two FAD2mutant alleles from SunOleic 95R into the genetic background of ICGV 6110, ICGV 6142 and ICGV 6420. In summary, 82 MABC and 387 MAS derived introgression lines (ILs) were developed using DNA markers with elevated oleic acid varying from 62 to 83%. Oleic acid increased by 0.5-1.1 folds, with concomitant reduction of linoleic acid by 0.4-1.0 folds and palmitic acid by 0.1-0.6 folds among ILs compared to recurrent parents. Finally, high oleate ILs, 27 with high oil (53.0-57.9%), and 28 ILs with low oil content (42.4-49.9%) were selected that may be released for cultivation upon further evaluation.
 
Publisher Elsevier
 
Date 2016-01
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/8986/1/1-s2.0-S0168945215300455-main.pdf
Janila, P and Pandey, M K and Shasidhar, Y and Variath, M T and Sriswathi, M and Khera, P and Manohar, S S and Nagesh, P and Vishwakarma, M K and Mishra, G P and Radhakrishnan, T and Manivannan, N and Dobariya, K L and Vasanthi, R P and Varshney, R K (2016) Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes. Plant Science. pp. 203-213. ISSN 0168-9452