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SSR Markers Linked with Alectra vogelii Resistance in Cowpea (Vigna unguiculata (L.) Walp)

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Relation http://oar.icrisat.org/9230/
 
Title SSR Markers Linked with Alectra vogelii Resistance in Cowpea (Vigna unguiculata (L.) Walp)
 
Creator Omoigui, L O
Ugba, M S
Bello, L L
Gowda, B S
Timlo, M P
Motagi, B N
 
Subject Food legumes
 
Description Cowpea [Vigna unguiculata (L.) Walp.] is an important
food legume grown in tropical and subtropical regions
of the world, primarily in Sub-Saharan Africa. Despite
the importance of cowpea, yield on farmers' field is
still low due to variety of biotic and abiotic stresses that constrain its production. Among the biotic constraints, the parasitic flowering plant, Alectra voge/ii (Benth.) is one of the more formidable limitations to cowpea production in the dry Savannas of West and Central Africa, a region which accounts for over 64 % of world cowpea production. Several control measures
have been suggested for the control of the parasite.
These include: cultural practices, application of
ethylene chemicals, and host plant resistance. Among
these control measures, the use of resistance cultivars
appears to be the most attractive option to the
resource poor farmers in sub-Saharan Africa Breeding
resistance cultivars would be facilitated by markerassisted
selection (MAS). The objective of this study
was to identify molecular markers tightly linked to
Alectra resistance gene that would be useful in MAS
in breeding cowpea for resistance to Alectra vogelii.
F2 population of a single cross, Banjar (susceptible
parent) x B30 I (resistant parent) was screened for
reaction to Alectra using pot culture technique. DNA
was extracted from parental genotypes and F2 lines
from young leaves of plant at 14 days after planting
using FTA ® PlantSaver cards. 50 SSR cowpea, 40
SSR rice bean and 50 SSR asparagus bean primers,
previously reported to give amplification products
in cowpea, were used to screen DNA from B30 I
and Banjar for polymorphism. Of the 140 primers
screened 20 primers were polymorphic between B30 I
and Banjar and these were used in the technique of
BSA performed with DNA bulks of highly resistant
and highly susceptible F2 lines to select those that cosegregate
with the resistant gene. Two of the markers
(RB 16 from rice bean and CLM0356 from asparagus
bean) were found to be consistently associated with the
resistance gene. The utility of these two markers were
validated using 150 F21ines for marker segregation and
association analysis. Similarity index (SI) revealed
that these markers were closely linked (90.23%) with
Alectra resistance gene. Cluster analysis as depicted
by dendogram also showed a tight association (>0.75)
between these markers, suggesting that these markers
can be explored in MAS targeting breeding for Alectra
resistance in cowpea.
 
Date 2015-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/9230/1/SSR-Alectra%20resitance%20cowpea%28NGG2015%29%20pdf.pdf
Omoigui, L O and Ugba, M S and Bello, L L and Gowda, B S and Timlo, M P and Motagi, B N (2015) SSR Markers Linked with Alectra vogelii Resistance in Cowpea (Vigna unguiculata (L.) Walp). In: 5th International conference on Next Generation Genomics and Integrated Breeding for Crop Improvement (NGGIBCI-V), February 18 - 20, 2015, ICRISAT, Patancheru, Hyderabad.