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Promoting Utilization of Saccharum spp. Genetic Resources through Genetic Diversity Analysis and Core Collection Construction

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Relation http://oar.icrisat.org/9354/
http://dx.doi.org/10.1371/journal.pone.0110856
 
Title Promoting Utilization of Saccharum spp. Genetic Resources through Genetic Diversity Analysis and Core Collection Construction
 
Creator Nayak, S N
Song, J
Villa, A
Pathak, B
Ayala-Silva, T
Yang, X
Todd, J
Glynn, N C
Kuhn, D N
Glaz, B
Gilbert, R A
Comstock, J C
Wang, J
 
Subject Others
Genetics and Genomics
 
Description Sugarcane (Saccharum spp.) and other members of Saccharum spp. are attractive biofuel feedstocks. One of the two World Collections of Sugarcane and Related Grasses (WCSRG) is in Miami, FL. This WCSRG has 1002 accessions, presumably with valuable alleles for biomass, other important agronomic traits, and stress resistance. However, the WCSRG has not been fully exploited by breeders due to its lack of characterization and unmanageable population. In order to optimize the use of this genetic resource, we aim to 1) genotypically evaluate all the 1002 accessions to understand its genetic diversity and population structure and 2) form a core collection, which captures most of the genetic diversity in the WCSRG. We screened 36 microsatellite markers on 1002 genotypes and recorded 209 alleles. Genetic diversity of the WCSRG ranged from 0 to 0.5 with an average of 0.304. The population structure analysis and principal coordinate analysis revealed three clusters with all S. spontaneum in one cluster, S. officinarum and S. hybrids in the second cluster and mostly non-Saccharum spp. in the third cluster. A core collection of 300 accessions was identified which captured the maximum genetic diversity of the entire WCSRG which can be further exploited for sugarcane and energy cane breeding. Sugarcane and energy cane breeders can effectively utilize this core collection for cultivar improvement. Further, the core collection can provide resources for forming an association panel to evaluate the traits of agronomic and commercial importance.
 
Publisher Public Library of Science
 
Date 2014-10-21
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/9354/1/57.pdf
Nayak, S N and Song, J and Villa, A and Pathak, B and Ayala-Silva, T and Yang, X and Todd, J and Glynn, N C and Kuhn, D N and Glaz, B and Gilbert, R A and Comstock, J C and Wang, J (2014) Promoting Utilization of Saccharum spp. Genetic Resources through Genetic Diversity Analysis and Core Collection Construction. PLoS ONE, 09 (10). 01-12. ISSN 1932-6203