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Characterization of potential probiotic bacteria isolated from sorghum and pearl millet of the semi-arid tropics

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Relation http://oar.icrisat.org/9432/
http://dx.doi.org/10.5897/AJB2016.15212
10.5897/AJB2016.15212
 
Title Characterization of potential probiotic bacteria isolated from sorghum and pearl millet of the semi-arid tropics
 
Creator Kunchala, R
Banerjee, R
Mazumdar, S D
Durgalla, P
Srinivas, V
Gopalakrishnan, S
 
Subject Pearl Millet
Sorghum
 
Description The present study was conducted to isolate and characterize probiotic properties of bacteria isolated from flour and batter samples of sorghum and pearl millet. A total of five different selective media including plate count agar, yeast glucose chloramphenicol agar, Bifidobacterium agar, actinomycetes isolation agar and de ManRogosa and Sharpe agar were used and the most prominent bacteria (which were found abundantly in the plate) were isolated and maintained on the respective media slants at 4°C for further studies. The bacteria were characterized for various traits including Gram staining, morphology (color, size, shape, elevation, margin, form and surface), biochemistry (urease, catalase, oxidase, hydrogen sulphide, nitrogen reduction, gelatin liquefaction, starch hydrolysis and carbohydrate utilization), IMViC tests (indole, methyl red, Voges Proskauer and citrate utilization), probiotic potentials [acid (pH 2, 3), bile (0.5%), NaCl (6 and 9%)], phenol tolerance [0.4%], antibiotic tolerance (tetracycline, streptomycin, kanamycin, chloramphenicol, ciprofloxacin, ampicillin, penicillin, erythromycin and vancomycin) and antimicrobial activity against human pathogens (Escherichia coli, Staphylococcus aureus and Salmonella typhi). A total of nine probiotic bacterial isolates were short listed based on these traits. The sequences of 16s rDNA gene of the nine isolates were found matched with Bacillus subtilis (two isolates), Bacillus cereus (three isolates), Bacillus pumilus (one isolate), Bacillus amyloliquefaciens (one isolate), Sphingobacterium thalpophilum (one isolate) and Brevibacterium sp. (one isolate) in BLAST analysis. The sequences of the nine bacteria were submitted to NCBI and accession numbers obtained. This study indicated that the selected bacteria could be exploited to develop new probiotic foods.
 
Publisher Academic Journals
 
Date 2016-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/9432/1/sorghum%20and%20pearl%20millet%20semi-arid%20tropics.pdf
Kunchala, R and Banerjee, R and Mazumdar, S D and Durgalla, P and Srinivas, V and Gopalakrishnan, S (2016) Characterization of potential probiotic bacteria isolated from sorghum and pearl millet of the semi-arid tropics. African Journal of Biotechnology, 15 (16). pp. 613-621. ISSN 1684-5315