Genetic dissection of seed-iron and zinc concentrations in chickpea
OAR@ICRISAT
View Archive InfoField | Value | |
Relation |
http://oar.icrisat.org/9436/
http://dx.doi.org/10.1038/srep24050 10.1038/srep24050 |
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Title |
Genetic dissection of seed-iron and zinc concentrations in chickpea
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Creator |
Upadhyaya, H D
Bajaj, D Das, S Kumar, V Gowda, C L L Sharma, S Tyagi, A K Parida, S K |
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Subject |
Chickpea
Food legumes Genetics and Genomics |
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Description |
The SNP-based high-resolution QTL mapping mapped eight major genomic regions harbouring robust QTLs governing seed-Fe and Zn concentrations (39.4% combined phenotypic variation explained/PVE) on six chromosomes of an intra-specific high-density genetic linkage map (1.56 cM map-density). 24620 SNPs discovered from genome-wide GBS (genotyping-by-sequencing) and 13 known cloned Fe and Zn contents-related chickpea gene-orthologs were genotyped in a structured population of 92 sequenced desi and kabuli accessions. The large-scale 16591 SNP genotyping- and phenotyping-based GWAS (genome-wide association study) identified 16 genomic loci/genes associated (29% combined PVE) with seed-Fe and Zn concentrations. Of these, 11 trait-associated SNPs in the genes linked tightly with eight QTLs were validated by QTL mapping. The seed-specific expression, including pronounced differential-regulation of 16 trait-associated genes particularly in accessions/mapping individuals with contrasting level of seed-Fe and Zn contents was apparent. Collectively, the aforementioned rapid integrated genomic strategy led to delineate novel functional non-synonymous and regulatory SNP allelic-variants from 16 known/candidate genes, including three strong trait-associated genes (encoding late embryogenesis abundant and yellow stripe-like 1 protein, and vacuolar protein sorting-associated protein) and eight major QTLs regulating seed-Fe and Zn concentrations in chickpea. These essential inputs thus have potential to be deployed in marker-assisted genetic enhancement for developing nutritionally-rich iron/zinc-biofortified chickpea cultivars.
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Publisher |
Nature Publishing Group
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Date |
2016-04-11
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Rights |
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Identifier |
http://oar.icrisat.org/9436/1/srep24050.pdf
Upadhyaya, H D and Bajaj, D and Das, S and Kumar, V and Gowda, C L L and Sharma, S and Tyagi, A K and Parida, S K (2016) Genetic dissection of seed-iron and zinc concentrations in chickpea. Scientific Reports, 06 (24050). 01-12. ISSN 2045-2322 |
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