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Polyketide Family of Novel Antibacterial 7‑O‑Methyl-5′-hydroxy-3′- heptenoate−Macrolactin from Seaweed-Associated Bacillus subtilis MTCC 10403

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Relation http://eprints.cmfri.org.in/10783/
 
Title Polyketide Family of Novel Antibacterial 7‑O‑Methyl-5′-hydroxy-3′-
heptenoate−Macrolactin from Seaweed-Associated Bacillus subtilis
MTCC 10403
 
Creator Chakraborty, Kajal
Thilakan, Bini
Raola, Vamshi Krishna
 
Subject Biochemistry
 
Description Seaweed-associated heterotrophic bacterial communities were screened to isolate potentially useful antimicrobial
strains, which were characterized by phylogenetic analysis. The bacteria were screened for the presence of metabolite genes
involved in natural product biosynthetic pathway, and the structural properties of secondary metabolites were correlated with the
genes. Bioactivity-guided isolation of polyene antibiotic 7-O-methyl-5′-hydroxy-3′-heptenoate−macrolactin from Bacillus subtilis
MTCC10403 associated with seaweed Anthophycus longifolius using mass spectrometry and extensive 2D-NMR studies was
carried out. The newly isolated macrolactin compound is a bactericidal antibiotic with broad spectrum activity against human
opportunistic clinical pathogens. The biosynthetic pathway of 7-O-methyl-5′-hydroxy-3′-heptenoate−macrolactin by means of a
stepwise, decarboxylative condensation pathway established the PKS-assisted biosynthesis of the parent macrolactin and the sidechain
5-hydroxyhept-3-enoate moiety attached to the macrolactin ring system at C-7. Antimicrobial activity analysis combined
with the results of amplifying genes encoding for polyketide synthetase and nonribosomal peptide synthetase showed that
seaweed-associated bacteria had broad-spectrum antimicrobial activity. The present work may have an impact on the exploitation
of macrolactins for pharmaceutical and biotechnological applications.
 
Publisher American Chemical Society
 
Date 2014
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/10783/1/Polyketide%20family_Journal%20of%20Agricultural%20and%20Food%20Chemistry_Kajal%20Chakraborty_62_50_2014.pdf
Chakraborty, Kajal and Thilakan, Bini and Raola, Vamshi Krishna (2014) Polyketide Family of Novel Antibacterial 7‑O‑Methyl-5′-hydroxy-3′- heptenoate−Macrolactin from Seaweed-Associated Bacillus subtilis MTCC 10403. Journal of Agricultural and Food Chemistry, 62 (50). pp. 194-208.