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Concentration of C20-22 n-3 polyunsaturated fatty acids from Sardinella longiceps and fatty acid stabilization

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Relation http://eprints.cmfri.org.in/10785/
http://onlinelibrary.wiley.com/doi/10.1002/ejlt.201400629/
 
Title Concentration of C20-22 n-3 polyunsaturated fatty acids from
Sardinella longiceps and fatty acid stabilization
 
Creator Chakraborty, Kajal
Joseph, Deepu
Joseph, Dexy
 
Subject Bioactive compounds
Biochemistry
 
Description Long chain n-3 polyunsaturated fatty acids from the refined oil from Sardinella longiceps were
concentrated by low-temperature crystallization and urea complexation. Low-temperature crystallization
(4°C) using acetone:ethylacetate (7:3, v/v) and urea complexation with urea-to-fatty acid ratio of
4:1 (w/w) afforded fatty acid concentrate (FAC) with greater than 60% C20–22 n-3 fatty acids, and
significant increase in NMR olefinic proton integral (at d 5.2–5.6). Ethylacetate fractions of seaweeds,
Kappaphycus alvarezii, Hypnea musciformis, and Jania rubens, which possessed greater antioxidant
activities, were added to FAC individually, and in nine factorial combinations (0.5%, w/w). The
oxidative stabilities of these treatments were compared with FAC added with BHT and a-tocopherol in
an accelerated storage study. A synergistic effect of ethylacetate fractions from K. alvarezii, H.
musciformis, and J. rubens used at 0.1:0.2:0.2 (%, w/w) in preventing the oxidative degradation of FAC
was observed. No traces of aromatics (d 6–7) and aldehydes (d 9–10) were present in the 1H-NMR
spectra of FAC added with seaweed extracts after the accelerated storage study, demonstrating that the
seaweed extracts were able to prevent the formation of low molecular weight secondary oxidation
products in the unsaturated system during storage.
 
Date 2016
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/10785/1/European%20Journal%20of%20Lipid%20Science%20and%20Technology_118_2016_Kajal_Concentration.pdf
Chakraborty, Kajal and Joseph, Deepu and Joseph, Dexy (2016) Concentration of C20-22 n-3 polyunsaturated fatty acids from Sardinella longiceps and fatty acid stabilization. European Journal of Lipid Science and Technology, 118 (2). pp. 208-223.