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Simple surface modification techniques for immobilization of biomolecules on SU-8

DSpace at IIT Bombay

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Field Value
 
Title Simple surface modification techniques for immobilization of biomolecules on SU-8
 
Creator DEEPU, A
SAI, VVR
MUKHERJI, S
 
Subject wave-guide
photoresist
mems
dna
 
Description SU-8, an epoxy based negative photoresist polymer has found wide range of applications in the field of microfabrication based biosensors. SU-8 surfaces need to be modified in order to immobilize bioreceptors. We studied the possibility of grafting desired functional groups by means of simple chemical treatments under normal laboratory conditions. These chemical treatments involve the use of crosslinkers that are expected to react with epoxy groups or hydroxyl groups generated by acid/alkali treatment. Here, a comparison of the results obtained on surface modification using glycine and 11-mercapto undecanoic acid as crosslinkers is presented. Human Immunoglobin G (HIgG) was covalently immobilized to carboxylic acid on SU-8 surface using carbodiimide/succinimide chemistry. The activity of immobilized HIgG was verified by using fluorescence imaging of FITC tagged goat anti HIgG bound to the surface. Fluorescence imaging was used to determine the chemistry best suited to functionalize SU-8 surface for biosensor applications.
 
Publisher SPRINGER
 
Date 2011-10-22T02:29:00Z
2011-12-15T09:10:40Z
2011-10-22T02:29:00Z
2011-12-15T09:10:40Z
2009
 
Type Article; Proceedings Paper
 
Identifier JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE,20,25-28
0957-4530
http://dx.doi.org/10.1007/s10856-008-3471-9
http://dspace.library.iitb.ac.in/xmlui/handle/10054/14796
http://hdl.handle.net/100/1585
 
Source Symposium on Materials for Biomedical Applications,Bangalore, INDIA,
 
Language English