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Micro-miniaturized electrophoresis DNA separator using MEMS

DSpace at IIT Bombay

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Title Micro-miniaturized electrophoresis DNA separator using MEMS
 
Creator APTE, PR
MOHANTA, LK
VARTAK, AC
 
Subject microchannel
multi-channel
electrophoresis
miniaturization
dna separation
 
Description Conventional DNA separation procedures involve large sizes, high voltages and are unfit for large molecules. Proposed set-up constitutes two fold separation techniques, porous filter for fractionating strands on size and final run through microchannels (agarose, buffer solution) viscous enough for DNA electrophoresis. For arriving at the final set-up all the physical contradictions like voltage, viscosity of the fluid, length of the channel were analyzed. The physical set up consists around 20 microchannels (varying diameters) positioned at 500um (centre-to-centre spacing) assembling entire device within 1cm. Polymerase Chain Reaction (PCR) treated DNA assays, are fed to microchannel entrances. Mixture of DNA strands are then passed through magnetic filters. Filtering property of the filters is adjusted by regulating corresponding magnetic field strengths. Smallest strands pass through small pored filter, owing to high velocity (in electrophoresis), thus categorization being done. Proposal replaces conventional apparatus by miniaturized equipment, in ideal case disposable. Miniaturization reduced voltages requirement, solving high-voltage handling problems. Proposed apparatus can fractionate large (> 200kbp) molecules and even organic molecules.
 
Publisher SPIE-INT SOC OPTICAL ENGINEERING
 
Date 2011-10-24T01:31:34Z
2011-12-15T09:11:21Z
2011-10-24T01:31:34Z
2011-12-15T09:11:21Z
2005
 
Type Proceedings Paper
 
Identifier MICROFLUIDICS, BIOMEMS, AND MEDICAL MICROSYSTEMS III,5718,276-282
0-8194-5692-6
0277-786X
http://dx.doi.org/10.1117/12.590195
http://dspace.library.iitb.ac.in/xmlui/handle/10054/15295
http://hdl.handle.net/100/2018
 
Source Conference on Microfludics, BioMEMS, and Medical Microsystems III,San Jose, CA,JAN 24-26, 2005
 
Language English