Dissolved core alginate microspheres as "Smart-Tattoo" glucose sensors
DSpace at IIT Bombay
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Title |
Dissolved core alginate microspheres as "Smart-Tattoo" glucose sensors
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Creator |
CHAUDHARY, A
RAINA, M MCSHANE, MJ SRIVASTAVA, R |
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Subject |
affinity sensor
nanofilm coatings concanavalin-a blood-glucose binding oxidase encapsulation microcapsules performance biosensors |
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Description |
The feasibility of multilayer thin film coated dissolved-core alginate-templated microsphere sensors based on fluorescence resonance energy transfer and competitive binding, was explored in simulated interstitial fluid, using glucose as a model analyte. The glucose sensitivity was observed to be 0.89%/mM glucose with a linear response in the range of 0-50mM glucose. The sensor response was observed to be completely reversible in nature with a response time of 120 seconds. The system was further demonstrated to respond similarly using near-infrared dyes (Alexa Fluor-647-labeled dextran as donor and QSY-21-conjugated apo-GOx as acceptor) which exhibited a sensitivity of 0.94%/mM glucose with a linear response in range of 0-50mM glucose, making the sensor more amenable to in vivo use, when implanted in scattering tissue.
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Publisher |
IEEE
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Date |
2011-10-25T16:37:22Z
2011-12-15T09:12:01Z 2011-10-25T16:37:22Z 2011-12-15T09:12:01Z 2009 |
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Type |
Proceedings Paper
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Identifier |
EMBC: 2009 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-20,4098-4101
978-1-4244-3295-0 http://dspace.library.iitb.ac.in/xmlui/handle/10054/15769 http://hdl.handle.net/100/2428 |
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Source |
Annual International Conference of the IEEE-Engineering-in-Medicine-and-Biology-Society,Minneapolis, MN,SEP 03-06, 2009
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Language |
English
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