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A low voltage single cell electroporator with a microfabricated sense-porate aperture

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Title A low voltage single cell electroporator with a microfabricated sense-porate aperture
 
Creator SARKAR, A
MITRA, B
SHASTRY, A
WADIA, S
MULHERKA, R
LAL, R
 
Description We present a novel flow-type single cell electroporation (SCE) system for low voltage electroporation of biological cells. We have used a microfabricated silicon sense-porate aperture to detect and identify a cell by its impedance and then apply the optimum electric field on it. Incorporation of a fluorescent dye into mouse embryo fibroblast NIH-3T3 cells has been demonstrated with a cell survival rate or viability much higher than conventional macroelectroporators. This paper reports the principle, design and implementation of this system and the experimental results obtained using it.
 
Publisher IEEE
 
Date 2011-10-27T20:49:57Z
2011-12-15T09:12:48Z
2011-10-27T20:49:57Z
2011-12-15T09:12:48Z
2004
 
Type Proceedings Paper
 
Identifier MEMS 2004: 17TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST,375-378
0-7803-8265-X
1084-6999
http://dspace.library.iitb.ac.in/xmlui/handle/10054/16412
http://hdl.handle.net/100/2905
 
Source 17th IEEE International Conference on Micro Electro Mechanical Systems,Maastricht, NETHERLANDS,JAN 25-29, 2004
 
Language English