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Fluorescent and photoactivable probes in depth-dependent analysis of membranes

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Title Fluorescent and photoactivable probes in depth-dependent analysis of membranes
 
Creator LALA, AK
 
Subject lipid probes
membranes
chlorpromazine
depth-dependent
fluorescent
photoactivable
human-erythrocyte membrane
inter-molecular crosslinking
fluorenyl fatty-acids
tryptophan fluorescence
hydrophobic core
conformational probes
labeled phospholipids
transmembrane domain
glycophorin-a
acyl chains
 
Description This report summarizes our efforts towards depth-dependent analysis of membranes by design Of Suitable fluorescent and photoactivable lipid probes which can be incorporated into membranes. The objective of depth-dependent analysis has been two Cold. one to obtain information on lipid domains and other on transmembrane domains of membrane-bound proteins. In of increasing importance of lipid rafts and other localized domain and limited success in case of structure determination of membrane-bound proteins vis-a-vis their Soluble counterparts, it is tempting to rapidly attach fluorescent or photoactivable probes to lipids to get a probes where relatively little attention is paid to design of such probes, We have shown here how careful design of such probes is required to immobilize such probes in membranes for effective depth-dependent analysis of membranes. An effective design has become important when identification of putative transmembrane domains predicted primarily from the genome data based on hydropathy plots. often needs confirmation by contemporary methodology. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
 
Publisher ELSEVIER SCI IRELAND LTD
 
Date 2011-10-13T18:23:46Z
2011-12-15T09:16:03Z
2011-10-13T18:23:46Z
2011-12-15T09:16:03Z
2002
 
Type Review
 
Identifier CHEMISTRY AND PHYSICS OF LIPIDS,116,177-188
0009-3084
http://dx.doi.org/10.1016/S0009-3084(02)00026-9
http://dspace.library.iitb.ac.in/xmlui/handle/10054/13815
http://hdl.handle.net/100/2973
 
Language en