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Genetic transformation in aspergilli: tools of the trade

DSpace at IIT Bombay

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Title Genetic transformation in aspergilli: tools of the trade
 
Creator PRABHA, VL
PUNEKAR, NS
 
Subject aspergilli
aspergillus niger
transformation strategies
genetic markers
transformation vectors
gene function
dominant selectable marker
autonomously replicating plasmid
protein disulfide-isomerase
dna-mediated transformation
encoding nitrate reductase
hygromycin-b resistance
beta-tubulin gene
filamentous fungi
neurospora-crassa
argb gene
 
Description DNA-mediated transformation is a powerful tool that allows the introduction of specific genetic changes in an organism. Transformation of Aspergilli, acclaimed for their wide use in the industry, has been possible for about two decades now. Several basic and applied problems related to fungal biology have been addressed using this technique. Nonetheless, new markers and strategies for transformation are still being developed for these filamentous fungi. Different methods and markers that are currently available for the transformation of Aspergilli are summarized here. The review also brings out the importance of these transformation systems in analyzing fungal gene function. Aspects of Aspergillus niger transformation are selectively emphasized.
 
Publisher NATL INST SCIENCE COMMUNICATION
 
Date 2011-10-12T11:47:44Z
2011-12-15T09:16:06Z
2011-10-12T11:47:44Z
2011-12-15T09:16:06Z
2004
 
Type Review
 
Identifier INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,41(5)205-215
0301-1208
http://dspace.library.iitb.ac.in/xmlui/handle/10054/13763
http://hdl.handle.net/100/2998
 
Language en