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Label-free detection techniques for protein microarrays: prospects, merits and challenges

DSpace at IIT Bombay

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Field Value
 
Title Label-free detection techniques for protein microarrays: prospects, merits and challenges
 
Creator RAY, S
MEHTA, G
SRIVASTAVA, S
 
Subject carbon nanotubes
interferometry
label-free techniques
protein arrays
protein microarrays
spr
arrayed imaging reflectometry
recombinant antibody microarrays
scanning kelvin nanoprobe
atomic-force microscopy
c-reactive protein
real-time
biomolecular interactions
nanohole arrays
quantitative-analysis
nanowire nanosensors
 
Description Protein microarrays, on which thousands of discrete proteins are printed, provide a valuable platform for functional analysis of the proteome. They have been widely used for biomarker discovery and to study protein-protein interactions. The accomplishments of DNA microarray technology, which had enabled massive parallel studies of gene expression, sparked great interest for the development of protein microarrays to achieve similar success at the protein level. Protein microarray detection techniques are often classified as being label-based and label-free. Most of the microarray applications have employed labelled detection such as fluorescent, chemiluminescent and radioactive labelling. These labelling strategies have synthetic challenges, multiple label issues and may exhibit interference with the binding site. Therefore, development of sensitive, reliable, high-throughput, label-free detection techniques are now attracting significant attention. Label-free detection techniques monitor biomolecular interactions and simplify the bioassays by eliminating the need for secondary reactants. Moreover, they provide quantitative information for the binding kinetics. In this article, we will review several label-free techniques, which offer promising applications for the protein microarrays, and discuss their prospects, merits and challenges.
 
Publisher WILEY-V C H VERLAG GMBH
 
Date 2011-10-12T12:22:52Z
2011-12-15T09:16:07Z
2011-10-12T12:22:52Z
2011-12-15T09:16:07Z
2010
 
Type Review
 
Identifier PROTEOMICS,10(4)731-748
1615-9853
http://dx.doi.org/10.1002/pmic.200900458
http://dspace.library.iitb.ac.in/xmlui/handle/10054/13769
http://hdl.handle.net/100/3004
 
Language en