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Cell-free synthesis-based protein microarrays and their applications

DSpace at IIT Bombay

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Field Value
 
Title Cell-free synthesis-based protein microarrays and their applications
 
Creator CHANDRA, H
SRIVASTAVA, S
 
Subject cell-free expression
multiple spotting technique
nucleic acid programmable protein array
protein in situ array
protein microarrays
high-throughput identification
in-vitro translation
free expression
free systems
arrays
dna
discovery
generation
antigens
microchips
 
Description The entire complement of proteins expressed by the genome of an organism under a set of defined conditions represents the proteome. The need for rapid, efficient, and high-throughput technologies to understand the proteome of organisms have resulted in the development of protein microarrays. Traditional, cell-based technologies for the generation of protein arrays involve laborious and protracted procedures, which also pose several other technical problems. These limitations have been suitably overcome by cell-free expression systems that carry out rapid, in situ synthesis of proteins from their corresponding DNA templates. This article provides an overview of commonly used cell-free systems that have been used for the generation of protein microarrays, with special emphasis on the diverse applications of cell-free synthesis-based protein microarrays.
 
Publisher WILEY-V C H VERLAG GMBH
 
Date 2011-10-12T12:39:44Z
2011-12-15T09:16:07Z
2011-10-12T12:39:44Z
2011-12-15T09:16:07Z
2010
 
Type Review
 
Identifier PROTEOMICS,10(4)717-730
1615-9853
http://dx.doi.org/10.1002/pmic.200900462
http://dspace.library.iitb.ac.in/xmlui/handle/10054/13772
http://hdl.handle.net/100/3007
 
Language en