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Analyzing metabolic variations in different bacterial strains, historical perspectives and current trends - example e. Coli

DSpace at IIT Bombay

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Title Analyzing metabolic variations in different bacterial strains, historical perspectives and current trends - example e. Coli
 
Creator SHILOACH, J
RESHAMWALA, S
NORONHA, SB
NEGRETE, A
 
Subject ionization mass-spectrometry
central carbon metabolism
escherichia-coli
flux analysis
liquid-chromatography
quantitative-analysis
acetate accumulation
expression patterns
glucose-utilization
growth
 
Description The analysis of metabolic differences in bacterial strains is a useful tool for the development of strains with desired growth and production properties. Several methods are available for the evaluation and understanding of the differences: Biochemical methods to measure metabolites concentration and enzyme activity, mathematical methods to analyze metabolic fluxes through the various pathways, proteomic methods to identify expressed proteins, and genomic methods to detect and measure gene expression. A combination of the various methods is required to obtain a comprehensive understanding of metabolic activities. The genomic methods provide substantial amount information on global gene expression but do not always reflect the actual activity of the individual components. The review focuses on the different methodologies and their use, as well as historical overview of the evaluation of the differences between Escherichia coil K and E. coli B.
 
Publisher CURRENT BIOLOGY LTD
 
Date 2011-10-14T11:28:02Z
2011-12-15T09:16:19Z
2011-10-14T11:28:02Z
2011-12-15T09:16:19Z
2010
 
Type Review
 
Identifier CURRENT OPINION IN BIOTECHNOLOGY,21(1)21-26
0958-1669
http://dx.doi.org/10.1016/j.copbio.2010.01.031
http://dspace.library.iitb.ac.in/xmlui/handle/10054/13906
http://hdl.handle.net/100/3107
 
Language en