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Disruption of galactokinase signature sequence in Gal3p of Saccharomyces cerevisiae does not lead to loss of signal transduction function

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Title Disruption of galactokinase signature sequence in Gal3p of Saccharomyces cerevisiae does not lead to loss of signal transduction function
 
Creator MURTHY, TVS
BHAT, PJ
 
Subject galactose induction
yeast
protein
pathway
gal80p
genes
activation
gal3
galactokinase
galactose
gal80
signal transducer
gal regulon
deletion analysis
 
Description Gal3p of Saccharomyces cerevisiae is a 520-amino-acid residue protein, which activates the GAL genes in the presence of galactose by relieving the repression of Gal80p, It shows significant amino acid sequence homology to galactokinases but does not possess galactokinase activity. Deletion mutants of Gal3p were generated to identify the role of N-terminal amino acid residues required for function. The mutant versions of Gal3p could be detected on a Western blot. The Gal3p mutant lacking N-terminal 50-amino-acid residues which is disrupted for galactokinase signature sequence was found to be functional. These results suggest that the evolutionarily conserved galactokinase signature sequence present in known galactokinases may not have a role in Gal3p function. (C) 2000
 
Publisher ACADEMIC PRESS INC
 
Date 2011-07-12T12:13:50Z
2011-12-26T12:48:52Z
2011-12-27T05:34:21Z
2011-07-12T12:13:50Z
2011-12-26T12:48:52Z
2011-12-27T05:34:21Z
2000
 
Type Article
 
Identifier BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 273(3), 824-828
0006-291X
http://dx.doi.org/10.1006/bbrc.2000.3015
http://dspace.library.iitb.ac.in/xmlui/handle/10054/3322
http://hdl.handle.net/10054/3322
 
Language en