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Effect of mycolic acid on surface activity of binary surfactant lipid monolayers

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Title Effect of mycolic acid on surface activity of binary surfactant lipid monolayers
 
Creator CHIMOTE, G
BANERJEE, R
 
Subject respiratory-distress-syndrome
pulmonary surfactant
dipalmitoylphosphatidylcholine monolayers
mycobacterium-tuberculosis
water-interface
film formation
palmitic acid
lung extracts
cholesterol
dysfunction
lung surfactant
monolayer
isotherm
mycolic acid
dppc
tuberculosis
phospholipid
 
Description In pulmonary tuberculosis, Mycobacterium tuberculosis lies in close physical proximity to alveolar surfactant. Cell walls of the mycobacteria contain loosely bound, detachable surface-active lipids. In this study, the effect of mycolic acid (MA), the most abundant mycobacterial cell wall lipid, on the surface activity of phospholipid mixtures from lung surfactant was investigated using Langmuir monolayers and atomic force microscopy (AFM). In the presence of mycolic acid, all the surfactant lipid mixtures attained high minimum surface tensions (between 20 and 40 mN/m) and decreased surface compressibility moduli < 50 mN/m. AFM images showed that the smooth surface topography of surfactant lipid monolayers was altered with addition of MA. Aggregates with diverse heights of at least two layer thicknesses were found in the presence of mycolic acid. Mycolic acids could aggregate within surfactant lipid monolayers and result in disturbed monolayer surface activity. The extent of the effect of mycolic acid depended on the initial state of the monolayer, with fluid films of DPPC-POPC and DPPC-CHOL being least affected. The results imply inhibitory effects of mycolic acid toward lung surfactant lipids and could be a mechanism of lung surfactant dysfunction in pulmonary tuberculosis. (C) 2008
 
Publisher ACADEMIC PRESS INC ELSEVIER SCIENCE
 
Date 2011-07-12T14:59:36Z
2011-12-26T12:48:56Z
2011-12-27T05:34:29Z
2011-07-12T14:59:36Z
2011-12-26T12:48:56Z
2011-12-27T05:34:29Z
2008
 
Type Article
 
Identifier JOURNAL OF COLLOID AND INTERFACE SCIENCE, 328(2), 288-298
0021-9797
http://dx.doi.org/10.1016/j.jcis.2008.08.022
http://dspace.library.iitb.ac.in/xmlui/handle/10054/3360
http://hdl.handle.net/10054/3360
 
Language en