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Molecular characterization reveals that YMR278w encoded protein is environmental stress response homologue of Saccharomyces cerevisiae PGM2

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Title Molecular characterization reveals that YMR278w encoded protein is environmental stress response homologue of Saccharomyces cerevisiae PGM2
 
Creator TIWARI, A
BHAT, JP
 
Subject budding yeast
phosphoglucomutase
gene
glucose
cells
uridine
identification
inactivation
purification
synthase
phosphoglucomutase
phosphoribomutase
enzyme evolution
 
Description The uncharacterized ORF YMR278w of Saccharomyces cerevisiae is a member of D-phosphohexomutase super family, annotated as phosphoribomutase. In order to evaluate its functional role, we cloned, over-expressed and purified YMR278w protein. The protein product of YMR278w exhibits phosphoglucomutase activity. S158T mutant derivative of YMR278w protein lost phosphoglucomutase activity. Purified YMR278w protein has higher K-m for glucose-1-phosphate compared to other known phosphoglucomutases. Trehalose content was reduced in YMR278w disruptant as compared to the wild type strain. Based on the above results we suggest that YMR278w encodes phosphoglucomutase and not phosphoribomutase. (C) 2007
 
Publisher ACADEMIC PRESS INC ELSEVIER SCIENCE
 
Date 2011-07-12T16:08:24Z
2011-12-26T12:48:58Z
2011-12-27T05:34:31Z
2011-07-12T16:08:24Z
2011-12-26T12:48:58Z
2011-12-27T05:34:31Z
2008
 
Type Article
 
Identifier BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 366(2), 340-345
0006-291X
http://dx.doi.org/10.1016/j.bbrc.2007.11.065
http://dspace.library.iitb.ac.in/xmlui/handle/10054/3374
http://hdl.handle.net/10054/3374
 
Language en