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Phosphinothricin resistance in Aspergillus niger and its utility as a selectable transformation marker

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Title Phosphinothricin resistance in Aspergillus niger and its utility as a selectable transformation marker
 
Creator AHUJA, M
PUNEKAR, NS
 
Subject l-methionine sulfoximine
amino-acid oxidase
glutamine-synthetase
neurospora-crassa
genetic-transformation
bialaphos resistance
chlamydomonas-reinhardtii
manganese peroxidase
herbicide-resistant
glufosinate
 
Description Aspergillus niger is moderately susceptible to inhibition by phosphinothricin (PPT)-a potent inhibitor of glutamine synthetase. This growth inhibition was relieved by L-glutamine. PPT inhibited A. niger glutamine synthetase in vitro (K-I, 54 mu M) and the inhibition was competitive with L-glutamate. The bar gene, imparting resistance to PPT, was successfully exploited as a dominant marker to transform this fungus. Very high PPT concentrations were required in the overlay for selection. Apart from bar transformants, colonies spontaneously resistant to PPT were frequently encountered on selection media. Reasons for such spontaneous resistance, albeit of moderate growth phenotype, were sought using one such isolate (SRPPT). The SRPPT isolate showed a 2-3-fold decrease in its glutamate uptake rate. Elevated external glutamate levels further suppressed the PPT-induced growth inhibition. Cellular entry of PPT could be through the L-glutamate uptake system thereby accounting for the observed spontaneous resistant phenotype. These results were useful in the fine-tuning of bar-selection in A. niger. (C) 2008
 
Publisher ACADEMIC PRESS INC ELSEVIER SCIENCE
 
Date 2011-07-12T16:37:54Z
2011-12-26T12:48:59Z
2011-12-27T05:34:33Z
2011-07-12T16:37:54Z
2011-12-26T12:48:59Z
2011-12-27T05:34:33Z
2008
 
Type Article
 
Identifier FUNGAL GENETICS AND BIOLOGY, 45(7), 1103-1110
1087-1845
http://dx.doi.org/10.1016/j.fgb.2008.04.002
http://dspace.library.iitb.ac.in/xmlui/handle/10054/3383
http://hdl.handle.net/10054/3383
 
Language en