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Detection of an intermediate during unfolding of bacterial cell division protein FtsZ - Loss of functional properties precedes the global unfolding of FtsZ

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Title Detection of an intermediate during unfolding of bacterial cell division protein FtsZ - Loss of functional properties precedes the global unfolding of FtsZ
 
Creator SANTRA, MK
PANDA, D
 
Subject molten globule state
gtp hydrolysis
tubulin polymerization
escherichia-coli
binding
conformation
fluorescence
mechanism
site
 
Description Using environment- sensitive fluorescence of 1- anilinonaphthalene-8- sulfonic acid, polarization of fluorescein 5 '- isothiocyanate- labeled FtsZ, and far- UV circular dichroism spectroscopy, the chemical unfolding of FtsZ was found to proceed through two steps. The first step of the urea- induced unfolding produced an intermediate, which then unfolded at higher concentrations of urea. The intermediate state contains native- like secondary structure and much less tertiary structure compared with the native state. It is distinct from the native state as well as from the unfolded state. Similar to urea- induced unfolding of FtsZ, thermal unfolding of FtsZ also occurs in two steps. The midpoints for the first and second thermal unfolding transitions were found to be 38 +/- 4 and 77 +/- 5 degreesC, respectively. Further, the functional properties of FtsZ are extremely sensitive to urea, guanidium chloride, and sodium dodecyl sulfate. For example, 50% inhibition of the FtsZ assembly and GTP hydrolysis occurred at 0.1 and 0.2 M of urea, respectively. FtsZ lost its functional properties before any significant perturbation in the secondary or tertiary structure was detected by using several fluorescence techniques and far UV- CD indicating preferential local unfolding of the functional region( s). In addition, the unfolded FtsZ regains its ability to polymerize fully upon removal of urea. The data taken together suggest that FtsZ unfolds reversibly through a multistep process, and local responses that inhibit functional properties precede the global transition of FtsZ to the unfolded state.
 
Publisher AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
 
Date 2011-07-17T04:36:19Z
2011-12-26T12:50:07Z
2011-12-27T05:36:07Z
2011-07-17T04:36:19Z
2011-12-26T12:50:07Z
2011-12-27T05:36:07Z
2003
 
Type Article
 
Identifier JOURNAL OF BIOLOGICAL CHEMISTRY, 278(24), 21336-21343
0021-9258
http://dx.doi.org/10.1074/jbc.M301303200
http://dspace.library.iitb.ac.in/xmlui/handle/10054/4617
http://hdl.handle.net/10054/4617
 
Language en