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Length and composition analysis of the cytoplasmic, transmembrane and stem regions of human Golgi glycosyltransferases

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Title Length and composition analysis of the cytoplasmic, transmembrane and stem regions of human Golgi glycosyltransferases
 
Creator PATEL, RY
BALAJI, PV
 
Subject protein secondary structure
n-acetylglucosaminyltransferase-v
beta-galactoside alpha-2,6-sialyltransferase
membrane-spanning domain
fucosyl-transferase-iii
structure prediction
in-vivo
bovine beta-1,4-galactosyltransferase
cysteine residues
complex-formation
topogenesis
type ii membrane protein
membrane anchoring
disorder promoting residues
 
Description A dataset of experimentally characterized, human Golgi GlyTs with type II membrane topology was created. Based on the experimentally observed acceptor substrate preferences, the GlyTs were classified into five functional categories: biosynthesis of blood group antigens, glycolipids, N-glycans, O-glycans and glycosaminoglycans. The cytoplasmic, transmembrane and stem (CTS) regions were predicted and their length and composition were analyzed. The stem region of GlyTs involved in the biosynthesis of glycolipids and blood group antigens appear to have a shorter stem region compared to those GlyTs which participate in the biosynthesis of N- and O-linked glycans and glycosaminoglycans. The stem regions of all the GlyTs, irrespective of the functional category to which they belong, were found to be rich in disorder-promoting amino acid residues. Thus, the stem region is largely devoid of any regular secondary structure thereby facilitating its tethering role. A higher frequency of occurrence of basic amino acids is observed towards the N-terminus of the transmembrane domain and this is suggested to be important for topogenesis of these enzymes.
 
Publisher BENTHAM SCIENCE PUBL LTD
 
Date 2011-07-18T23:55:52Z
2011-12-26T12:50:53Z
2011-12-27T05:37:05Z
2011-07-18T23:55:52Z
2011-12-26T12:50:53Z
2011-12-27T05:37:05Z
2007
 
Type Article
 
Identifier PROTEIN AND PEPTIDE LETTERS, 14(6), 601-609
0929-8665
http://dspace.library.iitb.ac.in/xmlui/handle/10054/5108
http://hdl.handle.net/10054/5108
 
Language en