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A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea

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Title A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea
 
Creator MUKHERJEE, A
SANTRA, MK
BEURIA, TK
PANDA, D
 
Subject escherichia-coli ftsz
hydrostatic-pressure
elasmobranch fishes
binding-protein
teleost fishes
ribonuclease-a
tubulin
ring
stabilization
denaturation
ftsz
ftsz unfolding
osmolyte
protofilaments bundling
tmao
 
Description Assembly of FtsZ was completely inhibited by low concentrations of urea and its unfolding occurred in two steps in the presence of urea, with the formation of an intermediate [Santra MK & Panda D (2003) J Biol Chem278, 21336-21343]. In this study, using the fluorescence of 1-anilininonaphthalene-8-sulfonic acid and far-UV circular dichroism spectroscopy, we found that a natural osmolyte, trimethylamine N-oxide (TMAO), counteracted the denaturing effects of urea and guanidium chloride on FtsZ. TMAO also protected assembly and bundling of FtsZ protofilaments from the denaturing effects of urea and guanidium chloride. Furthermore, the standard free energy changes for unfolding of FtsZ were estimated to be 22.5 and 28.4 kJ.mol(-1) in the absence and presence of 0.6 m TMAO, respectively. The data are consistent with the view that osmolytes counteract denaturant-induced unfolding of proteins by destabilizing the unfolded states. Interestingly, TMAO was also found to affect the assembly properties of native FtsZ. TMAO increased the light-scattering signal of the FtsZ assembly, increased sedimentable polymer mass, enhanced bundling of FtsZ protofilaments and reduced the GTPase activity of FtsZ. Similar to TMAO, monosodium glutamate, a physiological osmolyte in bacteria, which induces assembly and bundling of FtsZ filaments in vitro[Beuria TK, Krishnakumar SS, Sahar S, Singh N, Gupta K, Meshram M & Panda D (2003) J Biol Chem278, 3735-3741], was also found to counteract the deleterious effects of urea on FtsZ. The results together suggested that physiological osmolytes may regulate assembly and bundling of FtsZ in bacteria and that they may protect the functionality of FtsZ under environmental stress conditions.
 
Publisher BLACKWELL PUBLISHING
 
Date 2011-07-19T03:12:33Z
2011-12-26T12:50:58Z
2011-12-27T05:37:14Z
2011-07-19T03:12:33Z
2011-12-26T12:50:58Z
2011-12-27T05:37:14Z
2005
 
Type Article
 
Identifier FEBS JOURNAL, 272(11), 2760-2772
1742-464X
http://dx.doi.org/10.1111/j.1742-4658.2005.04696.x
http://dspace.library.iitb.ac.in/xmlui/handle/10054/5157
http://hdl.handle.net/10054/5157
 
Language en