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DETERMINATION OF TOTAL PROTON RELEASE IN PURPLE MEMBRANE SUSPENSION BY UMBELLIFERONE FLUORESCENCE QUENCHING TECHNIQUE

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Title DETERMINATION OF TOTAL PROTON RELEASE IN PURPLE MEMBRANE SUSPENSION BY UMBELLIFERONE FLUORESCENCE QUENCHING TECHNIQUE
 
Creator SONAR, S
SINGH, AK
 
Subject halobacterium-halobium
photochemical cycle
bacteriorhodopsin
fragments
 
Description A technique for determining total proton release from purple membrane suspension under steady illumination has been described. Illuminated purple membrane is found to quench the fluorescence life-time of umbelliferone indicating the release of protons in the medium. Besides the "stoichiometric" release of protons from bacteriorhodopsin, there seems to be release of protons from sources other than protonated retinylidene Schiff base moiety also.
 
Publisher COUNCIL SCIENTIFIC INDUSTRIAL RESEARCH
 
Date 2011-07-20T15:19:45Z
2011-12-26T12:51:33Z
2011-12-27T05:37:18Z
2011-07-20T15:19:45Z
2011-12-26T12:51:33Z
2011-12-27T05:37:18Z
1992
 
Type Article
 
Identifier INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 29(3), 251-254
0301-1208
http://dspace.library.iitb.ac.in/xmlui/handle/10054/5531
http://hdl.handle.net/10054/5531
 
Language en