DETERMINATION OF TOTAL PROTON RELEASE IN PURPLE MEMBRANE SUSPENSION BY UMBELLIFERONE FLUORESCENCE QUENCHING TECHNIQUE
DSpace at IIT Bombay
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Title |
DETERMINATION OF TOTAL PROTON RELEASE IN PURPLE MEMBRANE SUSPENSION BY UMBELLIFERONE FLUORESCENCE QUENCHING TECHNIQUE
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Creator |
SONAR, S
SINGH, AK |
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Subject |
halobacterium-halobium
photochemical cycle bacteriorhodopsin fragments |
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Description |
A technique for determining total proton release from purple membrane suspension under steady illumination has been described. Illuminated purple membrane is found to quench the fluorescence life-time of umbelliferone indicating the release of protons in the medium. Besides the "stoichiometric" release of protons from bacteriorhodopsin, there seems to be release of protons from sources other than protonated retinylidene Schiff base moiety also.
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Publisher |
COUNCIL SCIENTIFIC INDUSTRIAL RESEARCH
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Date |
2011-07-20T15:19:45Z
2011-12-26T12:51:33Z 2011-12-27T05:37:18Z 2011-07-20T15:19:45Z 2011-12-26T12:51:33Z 2011-12-27T05:37:18Z 1992 |
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Type |
Article
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Identifier |
INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 29(3), 251-254
0301-1208 http://dspace.library.iitb.ac.in/xmlui/handle/10054/5531 http://hdl.handle.net/10054/5531 |
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Language |
en
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