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A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae

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Title A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae
 
Creator VERMA, M
BHAT, PJ
BHARTIYA, S
VENKATESH, KV
 
Subject cyclin-cdk complex
signal-transduction
quantitative model
tryptophan operon
gene-expression
galactose
yeast
transcription
protein
binding
gal4p binding sites
gene expression
nucleocytoplasmic shuttling
regulatory networks
saccharomyces cerevisiae
 
Description Cellular regulation is a result of complex interactions arising from DNA-protein and protein-protein binding, autoregulation, and compartmentalization and shuttling of regulatory proteins. Experiments in molecular biology have identified these mechanisms recruited by a regulatory network. Mathematical models may be used to complement the knowledge-base provided by in vitro experimental methods. Interactions identified by in vitro experiments can lead to the hypothesis of multiple candidate models explaining the in vivo mechanism. The equilibrium dissociation constants for the various interactions and the total component concentration constitute constraints on the candidate models. In this work, we identify the most plausible in vivo network by comparing the output response to the experimental data. We demonstrate the methodology using the GAL system of Saccharomyces cerevisiae for which the steady-state analysis reveals that Gal3p neither dimerizes nor shuttles between the cytoplasm and the nucleus.
 
Publisher BLACKWELL PUBLISHING LTD
 
Date 2011-07-19T05:13:23Z
2011-12-26T12:51:01Z
2011-12-27T05:37:19Z
2011-07-19T05:13:23Z
2011-12-26T12:51:01Z
2011-12-27T05:37:19Z
2004
 
Type Article
 
Identifier EUROPEAN JOURNAL OF BIOCHEMISTRY, 271(20), 4064-4074
0014-2956
http://dx.doi.org/10.1111/j.1432-1033.2004.04344.x
http://dspace.library.iitb.ac.in/xmlui/handle/10054/5184
http://hdl.handle.net/10054/5184
 
Language en