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Molecular characterization of MRG19 of Saccharomyces cerevisiae - Implication in the regulation of galactose and nonfermentable carbon source utilization

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Title Molecular characterization of MRG19 of Saccharomyces cerevisiae - Implication in the regulation of galactose and nonfermentable carbon source utilization
 
Creator KHANDAY, FA
SAHA, M
BHAT, PJ
 
Subject rna-polymerase
transcriptional regulation
signal-transduction
glucose repression
gal4 protein
yeast
gene
induction
activation
location
carbon metabolism
cyc1 repressor
gal genes
glucose repression
induction signal
transcriptional regulator
 
Description We have reported previously that multiple copies of MRG19 suppress GAL genes in a wild-type but not in a gal80 strain of Saccharomyces cerevisiae. In this report we how that disruption of MRG19 leads to a decrease in GAL induction when S. cerevisiae is induced with 0.02% but not with 2.0% galacto e. Disruption of MRG19 in a gal3 background ( this train shows long-term adaptation phenotype) further delays the GAL induction, supporting the notion that its function is important only under low inducing signals. As a corollary, disruption of MRG19 in a gal80 strain did not decrease the constitutive expression of GAL genes. These results suggest that MRG19 has a role in GAL regulation only when the induction signal is weak. Unlike the effect on GAL gene expression, disruption of MRG19 leads to de-repression of CYC1-driven beta-galactosidase activity. MRG19 disruptant also showed a twofold increase in the rate of oxygen uptake as compared with the wild-type train. ADH2, CTA1, DLD1, and CYC7 promoters that are active during nonfermentative growth did not show any de-repression of beta-galactosidase activity in the MRG19 disruptant. Western blot analysis indicated that MRG19 is a glucose repressible gene and is expressed in galactose and glycerol plus lactate. Experiments using green fluorescent protein fusion constructs indicate that Mrg19p is localized in the nucleus consistent with the presence of a consensus nuclear localization signal sequence. Based on the above results, we propose that Mrg19p is a regulator of galactose and nonfermentable carbon utilization.
 
Publisher BLACKWELL PUBLISHING LTD
 
Date 2011-07-19T05:33:03Z
2011-12-26T12:51:01Z
2011-12-27T05:37:19Z
2011-07-19T05:33:03Z
2011-12-26T12:51:01Z
2011-12-27T05:37:19Z
2002
 
Type Article
 
Identifier EUROPEAN JOURNAL OF BIOCHEMISTRY, 269(23), 5840-5850
0014-2956
http://dx.doi.org/10.1046/j.1432-1033.2002.03303.x
http://dspace.library.iitb.ac.in/xmlui/handle/10054/5187
http://hdl.handle.net/10054/5187
 
Language en