Record Details

Simultaneous saccharification and fermentation of cellulose to lactic acid

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title Simultaneous saccharification and fermentation of cellulose to lactic acid
 
Creator VENKATESH, KV
 
Subject enzymatic-hydrolysis
model
inhibition
products
kinetics
ethanol
batch
cellulose
lactic acid
t-reesei
l-bulgaricus
enzymatic saccharification
 
Description Batch experiments were carried out for conversion of cellulose to lactic acid by simultaneous saccharification and fermentation (SSF) at different pH values. Saccharification was carried out with a cellulase enzyme preparation from T. reesei and fermentation was by L. bulgaricus. A kinetic model was developed to simulate SSF by incorporating cellulase enzyme kinetics with growth kinetics of L. bulgaricus. The enzyme kinetics were fitted by a Michaelis-Menten type equation, while the growth kinetics of L. bulgaricus were represented by a cybernetic-type model incorporating lactate and undissociated lactic acid inhibition term. The saccharification of cellulose was rate-limiting above pH 5, while lactic acid fermentation was rate limiting below pH 5. The glucose concentration in the fermentation broth was lower than that in simple saccharification (SS) at all pH values. Lactic acid productivity (0.45 g/l/h) was maximum at pH 5 and was higher than that of lactic acid productivity by SS followed by fermentation The kinetic model was able to predict the performance of SSF quite well. (C) 1997 .
 
Publisher ELSEVIER SCI LTD
 
Date 2011-07-22T13:02:58Z
2011-12-26T12:52:26Z
2011-12-27T05:37:42Z
2011-07-22T13:02:58Z
2011-12-26T12:52:26Z
2011-12-27T05:37:42Z
1997
 
Type Article
 
Identifier BIORESOURCE TECHNOLOGY, 62(3), 91-98
0960-8524
http://dx.doi.org/10.1016/S0960-8524(97)00122-3
http://dspace.library.iitb.ac.in/xmlui/handle/10054/6236
http://hdl.handle.net/10054/6236
 
Language en