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Steady state and time-resolved fluorescence investigation of the specific binding of two chlorin derivatives with human serum albumin

DSpace at IIT Bombay

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Title Steady state and time-resolved fluorescence investigation of the specific binding of two chlorin derivatives with human serum albumin
 
Creator PATEL, S
DATTA, A
 
Subject photodynamic therapy
warfarin-binding
site
ph
surfactant
drugs
model
p(6)
 
Description The specific binding of two model drugs for photodynamic therapy, namely chlorin P-6 and purpurin 18 in the vicinity of Sudlow's Site I of HSA has been investigated by monitoring the intrinsic fluorescence of single tryptophanyl residue and by competitive binding with warfarin. The distance from the tryptophanyl residue has been ascertained by FRET from Trp to the chlorins and has been found to indicate a binding to Sudlow's Site i. The principal driving force for the interaction is found to be the hydrophobic effect. The main mechanism of protein fluorescence quenching was static. Time-resolved fluorescence results of competitive binding with warfarin are found to confirm that they bind to the warfarin binding site.
 
Publisher AMER CHEMICAL SOC
 
Date 2011-07-14T21:41:52Z
2011-12-26T12:47:38Z
2011-12-27T05:37:46Z
2011-07-14T21:41:52Z
2011-12-26T12:47:38Z
2011-12-27T05:37:46Z
2007
 
Type Article
 
Identifier JOURNAL OF PHYSICAL CHEMISTRY B, 111(35), 10557-10562
1520-6106
http://dx.doi.org/10.1021/jp072544u
http://dspace.library.iitb.ac.in/xmlui/handle/10054/4076
http://hdl.handle.net/10054/4076
 
Language en