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Analysis of protein folding using polarity-sensitive fluorescent probes

DSpace at IIT Bombay

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Title Analysis of protein folding using polarity-sensitive fluorescent probes
 
Creator JOHN, B
D'SILVA, PR
LALA, AK
 
Subject molten globule
guanidine-hydrochloride
alpha-lactalbumin
state
intermediate
 
Description Polarity-sensitive fluorescent probes like 8-anilino-1-naphthalenesulphonate (ANS) and 1,1'-bis(4-anilino5-naphthalenesulphonic acid (bis-ANS) have been frequently used to detect equilibrium folding intermediates like the molten globule state, as the formation of the latter involves increase in hydrophobic exposure. The ability of these fluorescent probes to bind to the intermediate state thus provides a convenient method for detection of folding intermediates in a multiple-state folding transition. However, there is no convenient method available to quantitatively analyse the fluorescent changes detected as a function of denaturant concentration. Here we describe a new method for quantitative analysis that permits one to estimate free energy of folding and determine the relative population of the various states of protein in the solution.
 
Publisher CURRENT SCIENCE ASSN
 
Date 2011-07-21T05:11:33Z
2011-12-26T12:51:53Z
2011-12-27T05:38:37Z
2011-07-21T05:11:33Z
2011-12-26T12:51:53Z
2011-12-27T05:38:37Z
2001
 
Type Article
 
Identifier CURRENT SCIENCE, 80(2), 287-290
0011-3891
http://dspace.library.iitb.ac.in/xmlui/handle/10054/5793
http://hdl.handle.net/10054/5793
 
Language en