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Entrapment of live microbial cells in electropolymerized polyaniline and their use as urea biosensor

DSpace at IIT Bombay

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Title Entrapment of live microbial cells in electropolymerized polyaniline and their use as urea biosensor
 
Creator JHA, SK
KANUNGO, M
NATH, A
D'SOUZA, SF
 
Subject conducting polymer
immobilized enzymes
growth-rate
electrode
sensors
biomaterials
microtubules
performance
sulfonate)
aniline
immobilization
polyaniline
urea biosensor
whole cell
microbial biosensor
 
Description The lyophilized biomass of bacterium Brevibacterium ammoniagenes was immobilized in polystyrene sulphonate-polyaniline (PSS-PANI) conducting polymer on a Pt twin wire electrode by potentiostatic electropolymerization. The bacterial cells retained their viability as well as urease activity under entrapped state, as confirmed with bacterial live-dead fluorescent assay and enzymatic assays. The entrapped cells were visualized using scanning electron microscope. The immobilized cells were used as a source of unpurified urease to develop a conductometric urea biosensor. The catalytic action of urease in the sensor released ammonia, thereby causing an increase in the pH of the microenvironment. The pH dependant change in the resistivity of the polymer was used as the basis of sensing mechanism. The sensor response was linear over a range of 0-75 mM urea with a sensitivity of 0.125 mM(-1). The sensor could be reused for 12-15 independent measurements and was quite stable in dry as well as buffered storage condition at 4 degrees C for at least 7 days. (C) 2009
 
Publisher ELSEVIER ADVANCED TECHNOLOGY
 
Date 2011-07-21T15:45:43Z
2011-12-26T12:52:04Z
2011-12-27T05:39:01Z
2011-07-21T15:45:43Z
2011-12-26T12:52:04Z
2011-12-27T05:39:01Z
2009
 
Type Article
 
Identifier BIOSENSORS & BIOELECTRONICS, 24(8), 2637-2642
0956-5663
http://dx.doi.org/10.1016/j.bios.2009.01.024
http://dspace.library.iitb.ac.in/xmlui/handle/10054/5913
http://hdl.handle.net/10054/5913
 
Language en