Record Details

Microtubular conductometric biosensor for ethanol detection

DSpace at IIT Bombay

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Field Value
 
Title Microtubular conductometric biosensor for ethanol detection
 
Creator AJAY, AK
SRIVASTAVA, DN
 
Subject alcohol-dehydrogenase
sensor
polyaniline
films
nad+
alcohol sensor
conductometric sensor
polyaniline
adh
nadh
bipotentiostat
 
Description A conductometric sensor using microtubules of polyaniline as transducer cum immobilization matrix is reported, capable of detecting ethanol in liquid phase. Enzyme ADH (alcohol dehydrogenase) and its coenzyme NAD(+) have been used to improve the selectivity of the sensor. The sensor concept is based on the protonation of the polyaniline by the hydrogen ion produced in the enzyme-catalyzed reaction, leading to changes in the electrical conductance of the polyaniline. The sensor works well on the physiological pH, can detect ethanol as low as 0.02% (v/v) (0.092 M) and has a linear trend at par healthcare guidelines. The sensor responses were measured in various permutation and combination of enzyme and coenzyme concentrations and site of immobilization. The sensor shows minor interference with other functional groups and alcohols. The possible causes for such interference have been discussed. (C) 2007
 
Publisher ELSEVIER ADVANCED TECHNOLOGY
 
Date 2011-07-21T15:48:31Z
2011-12-26T12:52:04Z
2011-12-27T05:39:02Z
2011-07-21T15:48:31Z
2011-12-26T12:52:04Z
2011-12-27T05:39:02Z
2007
 
Type Article
 
Identifier BIOSENSORS & BIOELECTRONICS, 23(2), 281-284
0956-5663
http://dx.doi.org/10.1016/j.bios.2007.04.019
http://dspace.library.iitb.ac.in/xmlui/handle/10054/5914
http://hdl.handle.net/10054/5914
 
Language en