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Sodium channel modulating activity in a 5-conotoxin from an Indian marine snail

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Title Sodium channel modulating activity in a 5-conotoxin from an Indian marine snail
 
Creator SUDARSLAL, S
MAJUMDAR, S
RAMASAMY, P
DHAWAN, R
PAL, PP
RAMASWAMI, M
LALA, AK
SIKDAR, SK
SARMA, SP
KRISHNAN, KS
BALARAM, P
 
Subject receptor-site
rat-brain
conotoxin
peptides
toxins
venom
inactivation
blocker
delta-conotoxin
sodium channel
conus peptide
mass spectrometry
conus amadis
 
Description A 26 residue peptide (Am 2766) with the sequence CKQAGESCDIFSQNCCVG-TCAFICIE-NH2 has been isolated and purified from the venom of the molluscivorous snail, Conus amadis, collected off the southeastern coast of India. Chemical modification and mass spectrometric studies establish that Am 2766 has three disulfide bridges. C-terminal amidation has been demonstrated by mass measurements on the C-terminal fragments obtained by proteolysis. Sequence alignments establish that Am 2766 belongs to the delta-conotoxin family. Am 2766 inhibits the decay of the sodium current in brain rNav1.2a voltage-gated Na+ channel, stably expressed in Chinese hamster ovary cells. Unlike delta-conotoxins have previously been isolated from molluscivorous snails, Am 2766 inhibits inactivation of mammalian sodium channels. (C) 2003 on behalf of the Federation of European Biochemical Societies.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2011-07-26T17:44:52Z
2011-12-26T12:55:25Z
2011-12-27T05:39:50Z
2011-07-26T17:44:52Z
2011-12-26T12:55:25Z
2011-12-27T05:39:50Z
2003
 
Type Article
 
Identifier FEBS LETTERS, 553(1-2), 209-212
0014-5793
http://dx.doi.org/10.1016/S0014-5793(03)01016-0
http://dspace.library.iitb.ac.in/xmlui/handle/10054/6979
http://hdl.handle.net/10054/6979
 
Language en