Record Details

Magnetic field stimulation of hybridoma cells in vitro

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title Magnetic field stimulation of hybridoma cells in vitro
 
Creator DATTA, D
KUNDU, PK
DESHPANDE, A
 
Subject pulsing electromagnetic-fields
low-frequency
in-vitro
exposure
expression
culture
growth
cancer
antibody titer
hybridoma cells
magnetic field
 
Description Non-ionizing physical field interactions with cells, both in situ and in vitro, is of current interest globally. This is from various directions-starting from their abilities to induce permanent modifications in cell behavior in situ, through carcinogenesis and mutagenesis, to utilizing field effects for possibly enhancing the viable cell population in vitro. This results in parallel increase in some high-value, low-volume biochemical production. In the present study, screening experiments were carried out with a unique cell line-hybridoma (OKT3) (secreting monoclonal antibodies [MAbs] against T3 surface antigens of human peripheral CD4+ cells)-for a possible enhancement in the yield of extremely high value product (MAb). Overall, in the absence of any such data globally, there is apparently an urgent need for screening of such "field effects" on various other cell types in vitro for various reasons; e.g., low cost of manipulation, nonpolluting nature of interactions, distinct possibility of enhancement of produced biochemical titers, etc. In the present study, we observed various responses of the cell population both to magnetic fields alone and in combination with other known chemical stimulants of viable biomass (mono- and poly-lysine). Fifty hertz, 0.8 mT magnetic field and below, in conjunction with bulkier poly-lysine molecules, needs to be investigated further for a possible resonance-induced anti-interaction between these known mitogens and their cell surface receptors, which possibly could be extrapolated to other growth factor-receptor interactions in magnetic field environments, in situ.
 
Publisher MARCEL DEKKER INC
 
Date 2011-08-18T10:41:01Z
2011-12-26T12:55:42Z
2011-12-27T05:42:07Z
2011-08-18T10:41:01Z
2011-12-26T12:55:42Z
2011-12-27T05:42:07Z
2001
 
Type Article
 
Identifier ELECTRO- AND MAGNETOBIOLOGY, 20(3), 263-280
1061-9526
http://dx.doi.org/10.1081/JBC-100108569
http://dspace.library.iitb.ac.in/xmlui/handle/10054/9982
http://hdl.handle.net/10054/9982
 
Language en