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An Analysis of FtsZ Assembly Using Small Angle X-ray Scattering and Electron Microscopy

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Title An Analysis of FtsZ Assembly Using Small Angle X-ray Scattering and Electron Microscopy
 
Creator KUCHIBHATLA, A
RASHEED, ASA
NARAYANAN, J
BELLARE, J
PANDA, D
 
Subject bacterial-cell division
protein ftsz
fourier transformation
mechanism
tubulin
glutamate
polymerization
cytokinesis
filaments
homolog
 
Description Small angle X-ray scattering (SAXS) was used for the first time to study the self-assembly of the bacterial cell division protein, FtsZ, with three different additives: calcium chloride, monosodium glutamate and DEAF-dextran hydrochloride in solution. The SAXS data were analyzed assuming a model form factor and also by a model-independent analysis using the pair distance distribution function. Transmission electron microscopy (TEM) was used for direct observation of the FtsZ filaments. By sectioning and negative staining with glow discharged grids, very high bundling as well as low bundling polymers were observed under different assembly conditions. FtsZ polymers formed different structures in the presence of different additives and these additives were found to increase the bundling of FtsZ protofilaments by different mechanisms. The combined use of SAXS and TEM provided us a significant insight of the assembly of FtsZ and microstructures of the assembled FtsZ polymers.
 
Publisher AMER CHEMICAL SOC
 
Date 2011-07-13T15:58:40Z
2011-12-26T12:47:57Z
2011-12-27T05:42:28Z
2011-07-13T15:58:40Z
2011-12-26T12:47:57Z
2011-12-27T05:42:28Z
2009
 
Type Article
 
Identifier LANGMUIR, 25(6), 3775-3785
0743-7463
http://dx.doi.org/10.1021/la8036605
http://dspace.library.iitb.ac.in/xmlui/handle/10054/3713
http://hdl.handle.net/10054/3713
 
Language en