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Shock wave based biolistic device for DNA and drug delivery

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Title Shock wave based biolistic device for DNA and drug delivery
 
Creator NAKADA, M
MENEZES, V
KANNO, A
HOSSEIN, SHR
TAKAYAMA, K
 
Subject high-velocity microprojectiles
gene-expression
tobacco
cells
invivo
shock wave
laser ablation
biolistic
dna/drug delivery
gene expression
 
Description A shock wave assisted biolistic (biological ballistic) device has been developed to deliver DNA/dr-ug-coated micro-projectiles into soft living targets. The device consists of an Nd:YAG laser, an optical setup to focus the laser beam and, a thin aluminum (Al) foil (typically 100 mu m thick) which is a launch pad for the micro-projectiles. The DNA/drug-coated micro-particles to be delivered are deposited on the anterior surface of the foil and the posterior surface of the foil is ablated using the laser beam with an energy density of about 32 x 10(9) W/cm(2). The ablation launches a shock wave through the foil that imparts an impulse to the foil surface, due to which the deposited particles accelerate and acquire sufficient momentum to penetrate soft targets. The device has been tested for particle delivery by delivering 1 gm size tungsten particles into liver tissues of experimental rats and in vitro test models made of gelatin. The penetration depths of about 90 and 800 mu m have been observed in the liver and gelatin targets, respectively. The device has been tested for in vivo DNA [encoding beta-glucuronidase (GUS) gene] transfer by delivering plasmid DNA-coated, 1-mu m size gold (Au) particles into onion scale, tobacco leaf and soybean seed cells. The GUS activity was detected in the onion, tobacco and soybean cells after the DNA delivery. The present device is totally non-intrusive in nature and has a potential to get miniaturized to suit the existing medical procedures for DNA and/or drug delivery.
 
Publisher JAPAN SOCIETY APPLIED PHYSICS
 
Date 2011-08-04T08:56:21Z
2011-12-26T12:54:38Z
2011-12-27T05:42:46Z
2011-08-04T08:56:21Z
2011-12-26T12:54:38Z
2011-12-27T05:42:46Z
2008
 
Type Article
 
Identifier JAPANESE JOURNAL OF APPLIED PHYSICS, 47(3), 1522-1526
0021-4922
http://dx.doi.org/10.1143/JJAP.47.1522
http://dspace.library.iitb.ac.in/xmlui/handle/10054/9305
http://hdl.handle.net/10054/9305
 
Language en