Record Details

Does the anesthetic 2,2,2-trifluoroethanol interact with bovine serum albumin by direct binding or by solvent-mediated effects? A calorimetric and spectroscopic investigation

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title Does the anesthetic 2,2,2-trifluoroethanol interact with bovine serum albumin by direct binding or by solvent-mediated effects? A calorimetric and spectroscopic investigation
 
Creator BANERJEE, T
KISHORE, N
 
Subject partially folded state
beta-sheet protein
tryptophan fluorescence
secondary structure
alpha-lactalbumin
trifluoroethanol
peptides
alcohols
stabilization
lactoglobulin
bovine serum albumin
2,2,2-trifluoroethanol
differential scanning calorimetry
isothermal titration calorimetry
spectroscopy
 
Description Thermal unfolding of bovine serum albumin (BSA) has been studied in the presence of 2,2,2-trifluoroethanol (TFE) using high-sensitivity microdifferential scanning calorimetry. Quantitative thermodynamic parameters accompanying the thermal transitions have been evaluated. TFE is observed to be a stabilizer or a destabilizer of the folded state of BSA depending on the pH. CD spectroscopy revealed an increase in the α-helical content of BSA and a decrease in the tertiary structure in the presence of increasing molalities of TFE. Isothermal titration calorimetric results do not indicate appreciable binding of the TFE molecules to BSA. TFE quenches the steady-state tryptophan fluorescence of BSA only at higher molalities and there is no effect on the tryptophan fluorescence at lower molalities. The calorimetric and spectroscopic results obtained in this work suggest that solvent-mediated effects dominate the interaction of TFE with BSA and the binding component may be very weak. Since the binding component is very weak, one of the possibilities of anesthetic action of TFE molecules on the actual targets may be through perturbation of the structural and dynamic properties of the lipid bilayer so that the function of crucial but unspecified membrane proteins is affected. © 2005 .
 
Publisher JOHN WILEY & SONS INC
 
Date 2011-08-04T13:37:50Z
2011-12-26T12:54:44Z
2011-12-27T05:42:59Z
2011-08-04T13:37:50Z
2011-12-26T12:54:44Z
2011-12-27T05:42:59Z
2005
 
Type Article
 
Identifier BIOPOLYMERS, 78(2), 78-86
0006-3525
http://dx.doi.org/10.1002/bip.20262
http://dspace.library.iitb.ac.in/xmlui/handle/10054/9379
http://hdl.handle.net/10054/9379
 
Language en