Horseradish peroxidase catalyzed degradation of industrially important dyes
DSpace at IIT Bombay
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Title |
Horseradish peroxidase catalyzed degradation of industrially important dyes
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Creator |
BHUNIA, A
DURANI, S WANGIKAR, PP |
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Subject |
waste-water
phanerochaete-chrysosporium removal azo phenols decolorization model horseradish peroxidase (hrp) dye degradation wastewater treatment |
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Description |
Horseradish peroxidase (HRP) is known to degrade certain recalcitrant organic compounds such as phenol and substituted phenols. Here, for the first time we have shown HRP to be effective in degrading and precipitating industrially important azo dyes. For Remazol blue, the enzyme activity was found to be far better at pH 2.5 than at neutral pH. In addition, Remazol blue acts as a strong competitive inhibitor of HRP at neutral pH. Horseradish peroxidase shows broad substrate specificity toward a variety of azo dyes. Kinetic constants (K-m(app) and V-max(app)) for two different dyes have been determined. In addition to providing a systematic analysis of the potential of HRP in degradation of dyes, this study opens up a new area on exploration of commercial dyes as inhibitors of enzymes. 2001 , Inc.
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Publisher |
JOHN WILEY & SONS INC
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Date |
2011-08-16T06:55:46Z
2011-12-26T12:54:48Z 2011-12-27T05:43:06Z 2011-08-16T06:55:46Z 2011-12-26T12:54:48Z 2011-12-27T05:43:06Z 2001 |
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Type |
Article
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Identifier |
BIOTECHNOLOGY AND BIOENGINEERING, 72(5), 562-567
0006-3592 http://dx.doi.org/10.1002/1097-0290(20010305)72:5<562::AID-BIT1020>3.3.CO;2-J http://dspace.library.iitb.ac.in/xmlui/handle/10054/9418 http://hdl.handle.net/10054/9418 |
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Language |
en
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