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Improvement in bioreactor productivities using free radicals: HOCl-induced overproduction of xanthan gum from Xanthomonas campestris and its mechanism

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Title Improvement in bioreactor productivities using free radicals: HOCl-induced overproduction of xanthan gum from Xanthomonas campestris and its mechanism
 
Creator RAO, YM
SURESHKUMAR, GK
 
Subject escherichia-coli
hypochlorous acid
pv campestris
stress
dna
polysaccharide
mutagenesis
resistance
responses
free radicals
adaptive response
xanthomonas campestris
soxs-dna binding
xanthan gum
 
Description Free-radical induction has been employed as a novel strategy to improve bioreactor productivity and, more specifically, the quality and productivity of xanthan gum from Xanthomonas campestris cultures. A 210% increase in xanthan yield and a 20% increase in viscosity (quality) resulted from HOCl (oxidant) treatment. The acetate mass fraction in xanthan gum decreased by 42% and its pyruvate mass fraction increased by 63% as a result of HOCl treatment. The growth rate was almost unaffected by HOCl treatment. A hypothesis to explain the mechanism of xanthan gum overproduction by free-radical induction has been formulated. The significant aspects of the hypothesis, such as SoxS protein binding to the promoter region of the gum gene and the consequent increase in mRNA concentrations, have been experimentally verified. (C) 2001 , Inc.
 
Publisher JOHN WILEY & SONS INC
 
Date 2011-08-16T07:16:50Z
2011-12-26T12:54:49Z
2011-12-27T05:43:07Z
2011-08-16T07:16:50Z
2011-12-26T12:54:49Z
2011-12-27T05:43:07Z
2001
 
Type Article
 
Identifier BIOTECHNOLOGY AND BIOENGINEERING, 72(1), 62-68
0006-3592
http://dx.doi.org/10.1002/1097-0290(20010105)72:1<62::AID-BIT9>3.3.CO;2-0
http://dspace.library.iitb.ac.in/xmlui/handle/10054/9425
http://hdl.handle.net/10054/9425
 
Language en