FLUORESCENCE STUDIES ON ANTHRYL BACTERIORHODOPSINS
DSpace at IIT Bombay
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Title |
FLUORESCENCE STUDIES ON ANTHRYL BACTERIORHODOPSINS
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Creator |
SINGH, AK
ROY, M |
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Subject |
hydrogen-bonded chain
halobacterium-halobium purple membrane light energy proton pump opsin shift mechanism protein photoisomerization photochemistry bacteriorhodopsin bacteriorhodopsin analogs fluorescence energy transfer |
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Description |
Fluorescence properties of bacteriorhodopsin analogues containing 3-methyl-5-(9'-anthryl)-2E,4E-pentadienal and 3,7-dimethyl-9-(9'-anthryl)-2E,4E,6E,8E-nonatetraenal chromophores as built-in fluorescence probes are described. Determination of fluorescence quantum yields, fluorescence quenching and energy transfer efficiencies in terms of the Forster's energy transfer between tryptophans (Trp) and the anthrylidene moiety indicates that Trp residues lie at a critical distance (R(o)) of ca. 27 angstrom from the chromophore bound at Lys-216 on the apo-protein.
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Publisher |
ELSEVIER SCIENCE SA LAUSANNE
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Date |
2011-07-29T07:20:11Z
2011-12-26T12:48:32Z 2011-12-27T05:43:21Z 2011-07-29T07:20:11Z 2011-12-26T12:48:32Z 2011-12-27T05:43:21Z 1991 |
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Type |
Article
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Identifier |
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 8(3), 325-335
1011-1344 http://dx.doi.org/10.1016/1011-1344(91)80089-Z http://dspace.library.iitb.ac.in/xmlui/handle/10054/7630 http://hdl.handle.net/10054/7630 |
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Language |
en
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