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Characterization of silanization and antibody immobilization on spin-on glass (SOG) surface

DSpace at IIT Bombay

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Title Characterization of silanization and antibody immobilization on spin-on glass (SOG) surface
 
Creator NAGARE, GD
MUKHERJI, S
 
Subject silicon
silanes
afm
sog
spin-on glass
silanization
bio-functionalization
antibody immobilization
 
Description Most embedded optical waveguide biosensors require low dielectric optical material. The prerequisites for such applications include the selection of an appropriate material and effective bio-functionalization. Spin-on glass (SOG) is one of the preferred polymers that can be easily deposited. Further, the chemical properties of the polymer make it amenable for bio-functionalization. This paper reports a detailed study of a protocol for immobilization of antibodies on SOG for possible immunobiosensor applications. The effect of silanization at different pH values on the number of free and usable amine sites was quantified by fluorimetry. Contact angle measurements, ellipsometry, AFM, and fluorescence microscopy was used for assessing the surface properties at various stages of preparation, functionalization and biomolecule immobilization. (C) 2008
 
Publisher ELSEVIER SCIENCE BV
 
Date 2011-07-24T12:42:52Z
2011-12-26T12:55:00Z
2011-12-27T05:43:30Z
2011-07-24T12:42:52Z
2011-12-26T12:55:00Z
2011-12-27T05:43:30Z
2009
 
Type Article
 
Identifier APPLIED SURFACE SCIENCE, 255(6), 3696-3700
0169-4332
http://dx.doi.org/10.1016/j.apsusc.2008.10.033
http://dspace.library.iitb.ac.in/xmlui/handle/10054/6426
http://hdl.handle.net/10054/6426
 
Language en