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A study of the effect of JB particles on Saccharomyces cerevisiae (yeast) cells by Raman spectroscopy

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Title A study of the effect of JB particles on Saccharomyces cerevisiae (yeast) cells by Raman spectroscopy
 
Creator BHOWMICK, TK
PYRGIOTAKIS, G
FINTON, K
SURESH, AK
KANE, SG
MOUDGIL, B
BELLARE, JR
 
Subject lung epithelial-cells
free-radicals
living cells
tissue
damage
microspectroscopy
identification
membranes
proteins
spectra
raman spectroscopy
cell-particle interaction
yeast
freeze fracture
bhasma
 
Description The mechanism of interaction of particulate matter with living system is not completely understood. Evaluation of the effect of particulate Indian traditional medicine JB(JB) on Saccharomyces cerevisiae (yeast) cells is the major focus of the present study. In India, JB is considered as a rejuvenating medicine and used for the treatment of diseases such as diabetes and age-related eye diseases, as well as a health promoting tonic by the traditional practitioners. In presence of JB, higher growth has been observed at the late stationary growth phase of yeast. Ultra-structure analysis using transmission electron microscopy (TEM) has shown that JB-treated yeast cells have better morphology over control in the late stationary growth phase. In this investigation, cellular response from yeast cells after interaction with JB particles was measured using Raman spectroscopy. Raman spectroscopy -a noninvasive tool to distinguish between particle-treated and untreated cells - revealed that treatment with JB is able to slow the degradation of cellular components (e.g. DNA, proteins and lipids) with the aging of yeast cells. Copyright (C) 2008 , Ltd.
 
Publisher JOHN WILEY & SONS LTD
 
Date 2011-08-16T18:21:25Z
2011-12-26T12:55:05Z
2011-12-27T05:43:40Z
2011-08-16T18:21:25Z
2011-12-26T12:55:05Z
2011-12-27T05:43:40Z
2008
 
Type Article
 
Identifier JOURNAL OF RAMAN SPECTROSCOPY, 39(12), 1859-1868
0377-0486
http://dx.doi.org/10.1002/jrs.2051
http://dspace.library.iitb.ac.in/xmlui/handle/10054/9583
http://hdl.handle.net/10054/9583
 
Language en