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Combined effects of carbon, nitrogen and phosphorus substrates on D-ribose production via transketolase deficient strain of Bacillus pumilus

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Title Combined effects of carbon, nitrogen and phosphorus substrates on D-ribose production via transketolase deficient strain of Bacillus pumilus
 
Creator SRIVASTAVA, RK
WANGIKAR, PP
 
Subject amino-acid-transport
rifamycin-b fermentation
pentose-phosphate pathway
subtilis mutant strain
escherichia-coli k-12
saccharomyces-cerevisiae
aspergillus-nidulans
l-serine
optimization
glutamicum
d-ribose
b. pumilus
d-glucose
ams
cas amino acids
phosphate
 
Description BACKGROUND: D-ribose, a five carbon sugar, can be produced via mutant strains with impairment in the non-oxidative part of the pentose phosphate pathway (PPP). D-ribose is an important intermediate in the pharmaceutical industry. This work focuses on the effect of various substrates on D-ribose production with a transketolase deficient strain of Bacillus pumilus IFO13322. RESULT: D-ribose titer is found to increase with a concomitant decrease in the yield (on a g product g(-1) substrate basis) with increasing glucose concentration. Likewise, higher concentration of ammonium sulfate led to lower product yield while glucose and corn steep liquor concentrations were kept unaltered. Among the complex nitrogen substrates, cas amino acids showed better product yield than corn steep liquor. When tested for supplements of defined amino acids, media supplemented with tyrosine, phenylalanine, tryptophan and histidine gave better yields and higher biomass growth. This is possibly due to the fact that mutant strain is an aromatic amino acids auxotroph owing to impairment in aromatic amino acid biosynthesis. Further, higher phosphate concentrations in the media led to greater biomass formation and lower D-ribose yield. CONCLUSIONS: At higher glucose or ammonium sulfate concentrations, a greater proportion of the carbon was channeled toward byproducts such as acetate, and acetoin and biomass formation. Thus, the relative rates of PPP and in turn the D-ribose yield were dependent on not only the carbon substrate concentration but also the nitrogen and phosphorus substrates. (C) 2008 Society of Chemical Industry.
 
Publisher JOHN WILEY & SONS LTD
 
Date 2011-08-16T19:21:50Z
2011-12-26T12:55:06Z
2011-12-27T05:43:42Z
2011-08-16T19:21:50Z
2011-12-26T12:55:06Z
2011-12-27T05:43:42Z
2008
 
Type Article
 
Identifier JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 83(8), 1110-1119
0268-2575
http://dx.doi.org/10.1002/jctb.1936
http://dspace.library.iitb.ac.in/xmlui/handle/10054/9599
http://hdl.handle.net/10054/9599
 
Language en