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Ethenyl indoles as neutral hydrophobic fluorescence probes

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Title Ethenyl indoles as neutral hydrophobic fluorescence probes
 
Creator SINGH, AK
HOTA, PK
 
Subject bovine serum-albumin
spectral properties
surfactants
proteins
binding
sites
bsa
serum albumin
ethenyl indoles
fluorescence
binding constant
fluorescence quenching
 
Description 3-(4-Nitrophenylethenyl-E)-NH-indole (1), 3-(4-nitrophenyl ethenyl-E)-N-acetylindole (2), and 3-(4-nitrophenylethenyl-E)-N-benzenesulfonylindole (3) are relatively less fluorescent in organic solvents, with fluorescence quantum yield (Phi(f)) in the range of 0.002 to 0.066 depending on the solvent polarity. However, in bovine serum albumin (BSA)-phosphate buffer, the fluorescence of these compounds gets drastically enhanced with Phi(f) in the range of 0.21 to 0.67, depending on the substituent on the indolic nitrogen atom. Additionally, linear increase in the fluorescence intensity of 2 and 3 occurs on increasing the BSA concentration. These fluorescence properties together with the neutral, hydrophobic nature of these compounds make these fluorophores good fluorescence probe for studying the micropolarity of proteins like BSA and in general the ligand-protein interactions. Copyright (c) 2007 , Ltd.
 
Publisher JOHN WILEY & SONS LTD
 
Date 2011-08-16T21:25:35Z
2011-12-26T12:55:09Z
2011-12-27T05:43:47Z
2011-08-16T21:25:35Z
2011-12-26T12:55:09Z
2011-12-27T05:43:47Z
2007
 
Type Article
 
Identifier JOURNAL OF PHYSICAL ORGANIC CHEMISTRY, 20(8), 624-629
0894-3230
http://dx.doi.org/10.1002/poc.1222
http://dspace.library.iitb.ac.in/xmlui/handle/10054/9626
http://hdl.handle.net/10054/9626
 
Language en