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Kinetics and reaction engineering of penicillin G hydrolysis

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Title Kinetics and reaction engineering of penicillin G hydrolysis
 
Creator KUMAR, R
SURESH, AK
SHANKAR, HS
 
Subject penicillin g
6-amino penicillanic acid
penicillin-g-acylase
diffusion-reaction
fed-batch operation
 
Description A general diffusion reaction model for immobilised biocatalysis has been developed. The model has been used to study the deacylation of penicillin G to 6-aminopenicillanic acid using two commercially available immobilised Penicillin acylases. The values of D-e/R(2) for the enzyme pellets have been estimated using data on uptake of 6-aminopenicillanic acid and phenylacetic acid by the enzyme pellets. The kinetic parameters of the model were individually estimated from a suitably designed set of experiments. The values of the Thiele modulus from the kinetic parameters so calculated have been found to be in the range 1.67 to 9.8 for the two enzymes studied, implying that diffusional effects cannot be ignored. The effect of such diffusional limitations on the overall rates and hence on the utilisation of the intrinsic kinetic ability of the enzyme has been demonstrated. This paper also reports on the implementation of the fed-batch strategy for this system. The proposed strategy, which involves maintaining the substrate concentration at the optimum value for a large part of the conversion, results in higher product concentrations than in batch operation, thereby reducing downstream procesing costs. The productivity was also shown to be considerably higher than for batch operation. Further, the ease of implementation of this mode of operation has been demonstrated.
 
Publisher JOHN WILEY & SONS LTD
 
Date 2011-08-16T22:16:11Z
2011-12-26T12:55:10Z
2011-12-27T05:43:50Z
2011-08-16T22:16:11Z
2011-12-26T12:55:10Z
2011-12-27T05:43:50Z
1996
 
Type Article
 
Identifier JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 66(3), 243-250
0268-2575
http://dx.doi.org/10.1002/(SICI)1097-4660(199607)66:3<243::AID-JCTB488>3.0.CO;2-O
http://dspace.library.iitb.ac.in/xmlui/handle/10054/9642
http://hdl.handle.net/10054/9642
 
Language en