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0-phthalic acid, a dead-end product in one of the two pathways of phenanthrene degradation in Pseudomonas sp strain PP2

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Title 0-phthalic acid, a dead-end product in one of the two pathways of phenanthrene degradation in Pseudomonas sp strain PP2
 
Creator KRISHNAN, S
PRABHU, Y
PHALE, PS
 
Subject putida ous82
gene-cluster
kp7
biodegradation
dioxygenase
metabolism
1-hydroxy-2-naphthoate
dehydrogenase
naphthalene
pseudomonas sp strain pp2
phenanthrene metabolism
1, 2-dihydroxy naphthalene
0-phthalic acid
1-hydroxy-2-naphthoic acid dioxygenase
 
Description Phenanthrene is degraded via either o-phthalic acid or 1, 2-dihydroxynaphthalene in bacteria. A soil isolate Pseudomonas sp. strain PP2 degrades phenanthrene as the sole source of carbon, but failed to utilize naphthalene [Prabhu and Phale (2003) Appl Microbiol Biotechnol 61:342-351]. Analysis of the phenanthrene-grown culture spent media of this strain by gas chromatography-mass spectrometry (GC-MS) showed accumulation of o-phthalic acid. The cell-free extract prepared from this strain showed activity of 1-hydroxy-2-naphthoic acid dioxygenase (1-H-2-NADO). The extract showed conversion of 1-hydroxy-2-naphthoic acid and 2-carboxybenzaldehyde to o-phthalic acid, as analyzed by thin layer chromatography and GC-MS. However, it failed to grow or respire on o-phthalic acid. These results suggest that besides 1, 2-dihydroxynaphthalene pathway, the strain has a truncated o-phthalic acid pathway for phenanthrene metabolism and excretes o-phthalic acid as a dead-end product, indicating the co-existence of two pathways. 1-H-2-NADO, the key enzyme of o-phthalic acid pathway is inducible, has pH optima of 7.5, does not require external addition of Fe(II) as a co-factor and is completely inhibited by 1,10-phenanthroline. Absence of product formation under anaerobic condition and stoichiometric consumption of 0.82 moles Of O-2 per mole of product formed confirmed the dioxygenase nature of the enzyme.
 
Publisher NATL INST SCIENCE COMMUNICATION
 
Date 2011-08-19T02:50:28Z
2011-12-26T12:56:01Z
2011-12-27T05:44:02Z
2011-08-19T02:50:28Z
2011-12-26T12:56:01Z
2011-12-27T05:44:02Z
2004
 
Type Article
 
Identifier INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 41(5), 227-232
0301-1208
http://dspace.library.iitb.ac.in/xmlui/handle/10054/10200
http://hdl.handle.net/10054/10200
 
Language en