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Cloning and use of sC as homologous marker for Aspergillus niger transformation

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Title Cloning and use of sC as homologous marker for Aspergillus niger transformation
 
Creator VARADARAJALU, LP
PUNEKAR, NS
 
Subject encoding atp-sulfurylase
penicillium-chrysogenum
sulfate assimilation
gene
system
aspergillus niger
sc gene
transformation
marker
atp sulfarylase activity
 
Description The sC sequence from Aspergillus niger was cloned and developed into a homologous marker system for genetic transformation. The coding region of the sC gene amplified by PCR from the A. niger genome was provided with Aspergillus nidulans expression signals (gpdA promoter and trpC terminator). This chimeric construct was used to successfully transform a spontaneous sC(-) isolate of A. niger to prototrophy. The transformants analyzed by Southern analysis showed integration of multiple copies of the transforming DNA. They also exhibited much higher ATP sulfurylase activity than the wild-type A. niger strain reinforcing the molecular data. This demonstrates the usefulness of the sC(niger) construct, driven by PgpdA, as a marker for A. niger transformation. (C) 2004
 
Publisher ELSEVIER SCIENCE BV
 
Date 2011-07-24T13:12:33Z
2011-12-26T12:56:04Z
2011-12-27T05:44:12Z
2011-07-24T13:12:33Z
2011-12-26T12:56:04Z
2011-12-27T05:44:12Z
2005
 
Type Article
 
Identifier JOURNAL OF MICROBIOLOGICAL METHODS, 61(2), 219-224
0167-7012
http://dx.doi.org/10.1016/j.mimet.2004.11.022
http://dspace.library.iitb.ac.in/xmlui/handle/10054/6434
http://hdl.handle.net/10054/6434
 
Language en