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Oxidative-stress-induced production of pyocyanin by Xanthomonas campestris and its effect on the indicator target organism, Escherichia coli

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Title Oxidative-stress-induced production of pyocyanin by Xanthomonas campestris and its effect on the indicator target organism, Escherichia coli
 
Creator RAO, YM
SURESHKUMAR, GK
 
Subject oryzae pv oryzae
monofunctional catalase
superoxide-dismutase
resistance
oxygen
gene
dna
pyocyanin
free radicals
hocl
oxidative stress
xanthomonas campestris
 
Description Pyocyanin, a potential antimicrobial agent, was secreted by Xanthomonas campestris. Treatments with agents causing oxidative stress in the organism caused up to 4.4-fold increase in specific pyocyanin production. Pyocyanin added in the extracellular space did not affect growth rate of X. campestris, but decreased maximum cell concentration and specific product formation. However, the growth of Escherichia coil, the indicator target organism, was affected by pyocyanin. There was also a significant increase in the intracellular reactive oxygen species (ROS) concentration and antioxidant enzyme [catalase, superoxide dismutase (SOD)] concentrations, in the presence of pyocyanin. The intracellular ROS concentrations in E coli formed upon exposure to pyocyanin, which is an indicator of the toxicity, was dependent on the growth phase of the organism. Studies with mutants of E. coil showed that intracellular ROS concentration was not significantly affected by the absence of the regulon OxyR, but, was significantly higher in cases when the regulon rpoS or the genes-katG or katE were absent.
 
Publisher NATURE AMERICA INC
 
Date 2011-08-19T09:46:19Z
2011-12-26T12:56:11Z
2011-12-27T05:44:28Z
2011-08-19T09:46:19Z
2011-12-26T12:56:11Z
2011-12-27T05:44:28Z
2000
 
Type Article
 
Identifier JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 25(5), 266-272
1367-5435
http://dx.doi.org/10.1038/sj.jim.7000067
http://dspace.library.iitb.ac.in/xmlui/handle/10054/10310
http://hdl.handle.net/10054/10310
 
Language en