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Axial resolution limit of a fiber-optic fluorescence probe

DSpace at IIT Bombay

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Title Axial resolution limit of a fiber-optic fluorescence probe
 
Creator BALAJI, J
GARAI, K
CHAKRABARTI, S
MAITI, S
 
Subject confocal reflectance microscope
correlation spectroscopy
fluctuations
biosensors
system
 
Description We examine the limit of spatial resolution achievable when a single optical fiber is used for excitation and collection of fluorescence from a bulk specimen. We calculate the probability of detecting a fluorescent particle as a function of its position relative to the fiber face, using excitation wavelength X, radius a, numerical aperture N.A., and the particle's fluorescence and absorbance spectra. Treating Rhodamine B as a model fluorescent analyte and using appropriate fiber parameters, we show that the maximum axial resolution (defined as the axial distance in a homogenous solution within which 50% of the detected signal originates) achievable is similar to10 mum. We experimentally measured the axial resolution for a 500-muM aqueous solution of Rhodamine B with lambda = 543 nm, a = 1.31 mum, and a N.A. of 0.16 and found good qualitative agreement with the calculation. (C) 2003 Optical Society of America.
 
Publisher OPTICAL SOC AMER
 
Date 2011-08-19T10:20:02Z
2011-12-26T12:56:11Z
2011-12-27T05:44:29Z
2011-08-19T10:20:02Z
2011-12-26T12:56:11Z
2011-12-27T05:44:29Z
2003
 
Type Article
 
Identifier APPLIED OPTICS, 42(19), 3780-3784
0003-6935
http://dx.doi.org/10.1364/AO.42.003780
http://dspace.library.iitb.ac.in/xmlui/handle/10054/10316
http://hdl.handle.net/10054/10316
 
Language en