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Interaction of weakly bound antibiotics neomycin and lincomycin with bovine and human serum albumin: biophysical approach

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Title Interaction of weakly bound antibiotics neomycin and lincomycin with bovine and human serum albumin: biophysical approach
 
Creator KESWANI, N
CHOUDHARY, S
KISHORE, N
 
Subject isothermal titration calorimetry
magnetic-resonance-spectroscopy
drug binding-sites
aminoglycosides
resistance
proteins
rna
binding of antibiotics to serum albumin
bovine serum albumin
isothermal titration calorimetry
lincomycin
neomycin
 
Description The thermodynamics of interaction of neomycin and lincomycin with bovine serum albumin (BSA) and human serum albumin (HSA) has been studied using isothermal titration calorimetry (ITC), in combination with UV-visible, steady state and time resolved fluorescence spectroscopic measurements. Neomycin is observed to bind weakly to BSA and HSA whereas lincomycin did not show any evidence for binding with the native state of these proteins, rather it interacts in the presence of surfactants. The ITC results suggest 1 : 1 binding stoichiometry for neomycin in the studied temperature range. The values of the van't Hoff enthalpy do not agree with the calorimetric enthalpy in the case of neomycin, suggesting conformational changes in the protein upon ligand binding, as well as with the rise in the temperature. Experiments at different ionic strengths, and in the presence of tetrabutyl ammonium bromide and surfactants suggest the predominant involvement of electrostatic interactions in the complexation process of neomycin with BSA and HSA, and non-specific interaction behaviour of lincomycin with these proteins.
 
Publisher OXFORD UNIV PRESS
 
Date 2011-08-22T06:36:15Z
2011-12-26T12:56:14Z
2011-12-27T05:44:37Z
2011-08-22T06:36:15Z
2011-12-26T12:56:14Z
2011-12-27T05:44:37Z
2010
 
Type Article
 
Identifier JOURNAL OF BIOCHEMISTRY, 148(1), 71-84
0021-924X
http://dx.doi.org/10.1093/jb/mvq035
http://dspace.library.iitb.ac.in/xmlui/handle/10054/10347
http://hdl.handle.net/10054/10347
 
Language en